Determination of Tryptophan and Glutamic Acid During Fermentation of Kiwi-based Milk by Different Combinations of Saccharomyces boulardii and Lactobacilli
The aim of the present study was to evaluate the synergy interactions between Saccharomyces boulardii and preselected strains of lactobacilli during fermentation of skimmed milk fortified with kiwi juice (4% v/v) regarding tryptophan and glutamic acid production. The low capacity of S. boulardii in internalizing different sources of nitrogen produced marginal amounts of ≤0.66 mg/L (tryptophan) and ≤8.26 mg/L (glutamic acid) whether the milk was free or fortified with kiwi juice. The distinct production of tryptophan and glutamic acid were observed when the formulations were inoculated with lactobacilli and S. boulardii together. And so, the increased production was greater as much as ≤5.40 mg/L (tryptophan) and ≤12.09 mg/L (glutamic acid) when Lb. casei 20975 had a chance to grow in the milk with and without added kiwi juice where the S. boulardii was present. Lactobacilli strains could exercise its proteolytic system through cleavage of the higher molecular weight nitrogenous compounds. Indicating the presence of S. boulardii in the formulations where Lb. plantarum JXJ (6-12) or Lb. fermentum F16 were grown, the produced tryptophan and glutamic acid were at rapid rate and much more than those observed for the formulations inoculated with S. boulardii and Lb. plantarum RS (35-11), Lb. casei LCS, or Lb. fermentum F9.
Phillips M, Kailasapathy K, Tran L. Viability of commercial probiotic cultures (L. acidophilus, Bifidobacterium sp., L. casei, L. paracasei and L. rhamnosus) in cheddar cheese. International journal of food microbiology 2006; 108: 276–280.
S. Lahtinen, A. C. Ouwehand, S. Salminen and A. von Wright, Lactic acid bacteria: microbiological and functional aspects. Ed. Boca Raton, Florida, United States: CRC Press, 2011.
Wu R, Wang W, Yu D, Zhang W, Li Y, Sun Z, Wu J, Meng H, Zhang H. Proteomics Analysis of Lactobacillus casei Zhang, a New Probiotic Bacterium Isolated from Traditional Home-made Koumiss in Inner Mongolia of China. Molecular & Cellular Proteomics 2009; 8: 2321–2338.
SILVA CFGD, SANTOS FL, SANTANA LRRD, SILVA MVL, CONCEIÇÃO TDA. Development and characterization of a soymilk Kefir-based functional beverage. Food Science and Technology 2018; 38: 543–550.
Gaon D, Garcia H, Winter L, Rodríguez N, Quintas R, Gonzalez SN, Oliver G. Effect of Lactobacillus strains and Saccharomyces boulardii on persistent diarrhea in children. Medicina (Buenos Aires) 2003; 63: 293–298.
Lourens-Hattingh A, Viljoen BC. Growth and survival of a probiotic yeast in dairy products. Food Research International 2001; 34: 791–796.
Van der Aa Kühle A, Skovgaard K, Jespersen L. In vitro screening of probiotic properties of Saccharomyces cerevisiae var. boulardii and food-borne Saccharomyces cerevisiae strains. International journal of food microbiology, 2005; 101: 29–39.
Parvez S, Malik KA, Ah Kang S, Kim H. Probiotics and their fermented food products are beneficial for health. Journal of applied microbiology 2006; 100: 1171–1185.
Di Cagno R, Coda R, De Angelis M, Gobbetti M. Exploitation of vegetables and fruits through lactic acid fermentation. Food Microbiology 2013; 33: 1–10.
García-Nebot MJ, Alegría A, Barberá R, Clemente G, Romero F. Addition of milk or caseinophosphopeptides to fruit beverages to improve iron bioavailability?. Food Chemistry 2010; 119: 141–148.
Vanhee LME, Goemé F, Nelis HJ, Coenye T. Quality control of fifteen probiotic products containing Saccharomyces boulardii. Journal of Applied Microbiology 2010; 109: 1745–1752.
Srinivas B, Rani GS, Kumar BK, Chandrasekhar B, Krishna KV, Devi TA, Bhima B. Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm. AMB Express 2017; 7: 2.
Ali AA. Isolation and Identification of lactic acid bacteria isolated from traditional drinking yoghurt in Khartoum State, Sudan. Current Research in Bacteriology 2011; 4: 16–22.
Zhao N, Zhang C, Yang Q, Guo Z, Yang B, Lu W, Li D, Tian F, Liu X, Zhang H. Selection of taste markers related to lactic acid bacteria microflora metabolism for Chinese traditional paocai: a gas chromatography–mass spectrometry-based metabolomics approach. Journal of agricultural and food chemistry 2016; 64: 2415–2422.
Kok J. Genetics of the proteolytic system of lactic acid bacteria. FEMS Microbiology Letters 1990; 87: 15-42.
Khalid NM, Marth EH. Proteolytic activity by strains of Lactobacillus plantarum and Lactobacillus casei. Journal of Dairy Science 1990; 73: 3068–3076.
Solieri L, Rutella GS, Tagliazucchi D. Impact of non-starter lactobacilli on release of peptides with angiotensin-converting enzyme inhibitory and antioxidant activities during bovine milk fermentation. Food microbiology 2015; 51: 108–116.
de Nadra MCM, Arena ME, Saguir FM. Nutritional requirements and amino acids utilization by lactic acid bacteria from wine-A short review. JOURNAL OF FOOD AGRICULTURE AND ENVIRONMENT 2003; 1: 76–79.
Ljungdahl PO, Daignan-Fornier B. Regulation of amino acid, nucleotide, and phosphate metabolism in Saccharomyces cerevisiae. Genetics 2012; 190: 885–929.
Zhao XQ, and Bai FW. Mechanisms of yeast stress tolerance and its manipulation for efficient fuel ethanol production. Journal of Biotechnology 2009; 144: 23–30.