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Influence of Olive Leaf Extract and Stem Cell Therapy on Hepatic Cellular Injury Induced by Gamma Radiation in Albino Rats: Histological, Histochemical and Immunohistochemical Study
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Volume 3, 2016
Issue 4 (July)
Pages: 63-73   |   Vol. 3, No. 4, July 2016   |   Follow on         
Paper in PDF Downloads: 52   Since Jul. 1, 2016 Views: 803   Since Jul. 1, 2016
Tamer M. M. Abu-Amara, Histology and Cytology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Abd EL Razek A. Meselhy, Anatomy and Embryology Department, Faculty of Medicine, Al-Azhar University, Assiut, Egypt.
Background: Free radicals are harmful molecules produced when our body breaks down foodparticles or via environmental factors like exposures to radiation. Free radicals can damage cells. Antioxidants such as olive leaf extract are substances that may protect our bodies against the effects of free radicals. Also, stem cell therapy is a new trend that can be used to treat these damaging effects. Aim of the work: The goal of this work is to study the radio-protective effect of olive leaf extract as a natural antioxidant and stem cell as a regenerative therapy on the liver of adult albino rats against the gamma radiation damaging effects. Material and Methods: The present study was carried out on 24 adult albino rats of local strain weighing 120±3 g which were randomly divided equally into Group 1(C, untreated negative control):the rats served as negative control (without any treatment for one month). Group 2 (I, Irradiated): The rats irradiated via exposing to 6 Gy delivered as a fractionated dose (2 Gy each 3 days). Group 3 (I+O, Irradiated+Olive leaf extract treatment): The rats had a combination of irradiation (in the same way like group 2) plus oral intake of olive leaf extract for one month. Group 4 (I+S, Irradiated+Stem cell therapy): The rats irradiated (in the same way like group 2) after single injection of mesenchymal stem cells (MSCs). Paraffin sections were prepared for histological, histochemical and immnuohistochemical studies. The data were statistically analysed. Results: Examined sections showed significant cellular injury in group 2 in comparison to the control group. Groups 3 and 4 showed obvious decrease in the pathological changes occurring in comparison with group 2. Conclusion: These results provide evidence that both olive leaf extract and stem cell therapy have radio-protective effect as they reduced the pathological cellular injuries in the liver induced by accumulated doses of radiation exposure. Further studies may be done to confirm these findings.
Radiation, Liver, Olive, Stem Cells, Regenerative Medicine and Histochemsitry
Tolman, KG., Fonseca, V., Tan, MH., and Dalpiaz, A. (2004): Narrativereview: hepatobiliary disease in type 2 diabetes mellitus. Ann Intern Med., 141: 946-956.
Steel, GG. (1996): From targets to genes: a brief history of radio sensitivity. Phys Med Biol., 41: 205-222.
Hall, EJ. (2000): A radiation biologist looks to the future. Int J RadiatOncolBiol Phys., 46: 1-2.
Samarth, RM., and Kumar, A. (2003): MenthaPiperia leaf extract provides protection against radiation induced chromosomal damage in bone marrow of mice. Indian J Exp Biol., 41: 229-237.
Bhosle, SM., Huilgol, NG., Mishra, KP. (2005): Enhancement of radiation induced oxidative stress and cytotoxicity in tumor cells by ellagic acid. ClinChimActa., 359: 89-100.
Vincent, EG. (2008): Protection against radiation-the second line of defense. http://www.vincegiuliano.name/PROTECTION%20AGAINST%20RADIATIONc.htm#_ftn1
Gerber, M., Boutron-Ruault, MC., Hercberg, S., Riboli, E., and Scalbert, A. (2002): Food and cancer: state of the art about the protective effect of fruits and vegetables. Bull Cancer., 89: 293-312.
El-Habit, OH. Saada, HN., Azab, KS., Abdel-Rahman, M., and El-Malah, DF. (2000): The modifying effect of B-carotene on gamma radiation-induced elevation reactions and genotoxicity in male rat. Mutat Res., 466: 179-186.
Omar, SH. (2010): Oleuropein in Olive and its pharmacological effects. Sci.Pharm., 78:133-154.
Visioli, F., Poli, A., Galli, C. (2002): Antioxidant and other biological activities of phenols from olives and olive oil. Med. Res. Rev., 22: 65–75.
Andreadou, I.., Iliodromitis, EK., Mikros, E., Constantinou, M., Agalias, A., Magiatis, P., Skaltsounis, AL., Kamber, E., Tsantili-Kakoulidou, A., and Kremastinos, DT. (2006): The olive constituent oleuropein exhibits anti-ischemic, antioxidative, and hypolipidemic effects in anesthetized rabbits. JNutr., 136: 2213–2219.
Visioli, F., and Galli, C. (2001): Antiatherogenic components of olive oil. Curr. Atheroscler. Rep., 3: 64–67.
Carluccio, MA., Siculella, L., Ancora, MA., Massaro, M., Scoditti, E., Storelli, C., Visioli, F., Distante, A., and DeCaterina, R. (2003): Olive oil and red wine antioxidant polyphenols inhibit endothelial activation: antiatherogenicproperties of Mediterranean diet phytochemicals. Arterioscler. Thromb. Vasc. Biol., 23(4):622-629.
Hamdi, HK., and Castellon, R. (2005): Oleuropein, a non-toxic olive iridoid, is an anti-tumor agent and cytoskeleton disruptor. Biochem. Biophys. Res. Commun., 334: 769–778.
Menendez, JA., Vazquez-Martin, A., Colomer, R., Brunet, J., Carrasco-Pancorbo, A., Garcia-Villalba, R., Fernandez-Gutierrez, A., and Segura-Carretero, A. (2007): Olive oil's bitter principle reverses acquired autoresistance to trastuzumab (Herceptin™) in HER2-overexpressing breast cancer cells. B.M.C. Cancer., 7: 80-87.
Furneri, PM., Marino, A., Saija, A., Ucella, N., and Bisignano, G.(2002): In vitro antimycoplasmal activity of oleuropein. Int. J. Antimicrob. Agents., 20: 293–296.
Caturla, N., Perez Fons, L., Estepa, A., and Micol, V. (2005): Differential effects of oleuropein, a biophenol from Oleaeuropaea, on anionic and zwiter ionic phospholipid model membranes. Chem. Phys. Lipids., 137: 2–17.
Fredrickson, WR. (2000): Method and composition for antiviral therapy with olive leaves. Global Veterinaria., 6:117-844.
Ma, SC., He, ZD., Deng, XL., But, PP., Ooi, VE., Xu, HX., Lee, SH., and Lee, SF. (2001):In vitro evaluation of secoiridoidglucosides from the fruits of Ligustrumlucidumas antiviral agents. Chem. Pharm. Bull., 49: 1471–1473.
Seo, GS. (2011): Stem cell properties of therapeutic potential. Korean J Gastroenterol., 58: 125-132. 19.
Shrihari, TG. (2011): Cancer Stem Cells - Therapeutic Boon! J Cancer SciTher., 3: 197-200. 20. Quante M.
Wang, TC. (2009): Stem cells in gastroenterology and hepatology. Nat Rev GastroenterolHepatol., 6: 724-737.
Navarro-Alvarez, N., Soto-Gutierrez, A., Kobayashi, N. (2009): Stem cell research and therapy for liver disease. Curr Stem Cell Res Ther., 4: 141-146.
Wainstein, J., Ganz, T., Boaz, M., Dayan, YB., Dolev, E., Kerem, Z., and Madar, Z. (2012): Olive leaf extract as a hypoglycemic agent in both human diabetic subjects and in rats. Journal of Medicinal Food: 15: 605–610.
Bancroft, D., and Gamble, M. (2008): Bancroft's Theory and Practice of Histological Techniques. 6th Ed. Elsevier, Churchill Livingst one, Edinburgh, Scotland., Pp 178-186, 221-224.
Hammer, Ø., Harper, DAT., Ryan, PD. (2001): PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica., 4(1): 9-15.
Jagetia, GC. (2007): Radioprotective potential of plants and herbs against the effects of ionizing radiation. J. Clin. Biochem. Nutr., 40:74-81.
Kunwar, A., Bansal, P., Kumar, SJ., Bag, PP., Paul, P., Reddy, ND., Kumbhare, LB., Jain, VK., Chaubey, RC., Unnikrishnan, MK., and Priyadarsini, KI. (2010): In vivo radioprotection studies of 3,3'-diselenodipropionic acid, a selenocystine derivative. Free Rad. Biolo. Medic., 48: 399–410.
Saada, HN., Ussama, ZS., Mahdy, AM. (2003): Effectiveness of Aloe vera on the antioxidant status of different tissue in irradiated rats.J. Pharmazie., 58:1.
Azab, KhSh. (2007): Modulation of radiation injuries in rats receiving multiple doses of Aloe Vera. Egypt. J. Rad. Sci. and Applic., 20:17-28.
Ammar, AA. (2009): Evaluation of the protective role of wheat germ oil in irradiated rats. Isotope Rad. Res., 41: 911-920.
El-Masry, FS and Saad, TM. (2005): Role of selenium and vitamin E in modification of radiation disorders in male albino rats: Isotope Rad. Res.37., 5: 1261-1273.
Agarwal, A., and Kale, RK. (2001): Radiation induced oxidative damage: mechanisms and significance. Ind. J. Exp. Boil., 39: 291–309.
Mrcillo, MA., Rucandio, MI., Santamaria, J. (2000): Effect of gamma irradiation and lipid peroxidation in rats. Cell Mol. Biol., 92: 337.
Dawson, LA., Normalle, D., Balter, JM., McGinn, CJ., Lawrence, TS., and Haken Ten, RK. (2002): Analysis of radiation induced liver disease using the Lyman NTCP model. Int. J. Rad. Oncol. Biol. Phys., 53: 810–821.
Koc, M., Taysi, S., Buyukokuroglu, ME., and Bakan, N. (2003): Melatonin protects rat liver against irradiation induced oxidative injury. J. Rad. Res., 44: 211–215.
Song, LH., Yan, HL., and Cai, DL. (2006): Protective effects of soy bean is of lavone against gamma irradiation induced damages in mice. J. Rad. Res. Tokyo., 47(2): 157–165.
Rezkm, RG. (2005): Modulatory role of AloeVera on gamma irradiation induced histological changes in different tissues of rats. Egypt. J. Rad. Sci., Applic., 18(1): 97-115.
Said, UZ., Rezk, RG., a, HN. (2005): Role of calcium pantothenate in recovery of radiation induced injury to mammalian organs. Egypt.J.Rad.Sci., Applic., 18(1):17-38.
Soliman, O., Abd El Maguid, A., Hafez, MN. (2007): Histological and histochemical studies on liver of rat subjected to synergistic effect of natural and synthetic food colorants and/or gamma irradiation. Egypt.J.Rad.Sci.Applic., 20(1): 195-214.
Kafafy, YA., Abu Gabal, HA., Roushdy, HM., Abu Sinna, GM., andAshry, OM. (2001): Bone marrow transplantation controlling hormonal and structural changes in radiation exposed pregnant mice and their developing embryos. Egypt. J. Rad. Sci. Applic., 14(1): 1–23.
Kropacova, K., Slovinska, L., Misurova, E. (2002): Cytogenetic changes in the liver of progeny of irradiated male rats. J. Rad. Res., 43: 125–133.
Rady, MI., Abu Nour, SM., Khalil, HM. (2008): Effect of gamma radiation on albino rat's liver and the possible protective role of β-carotene on the histological and ultra structural changes. Egypt. J. Rad. Sci., Applic., 21(2): 373-388.
Jirtle, RL., Anscher, MS., Alati, T. (1990): Radiation sensitivity of the liver. In: ''Advances in Radiation Biology''. Academic Press, San Diego. 14.
Majno, G., and Joris, I. (1996): Cells, tissues and diseases. In: "Principal of general patholgy". Part I, Cellular Patho. Blackwell Sci. 202- Yarmonenko, SP. (1998): In radiobiology of human and animals. Mir. Publisher, Moscow., 33.
Zavodnik, LB., Kravchuk, R., Artsukevich, A., Chumachenko, S., Sheibak, M., Ovchinnikov, V., and Buko, V. (2003): Dynamicsofstructural changes in rat liver after single dose of gamma irradiation. Rad. Biol. Radioecol., 43(6): 618.
Guryey, DV. (2005): Histologic assessment of regenerating rat liver under low-dose rate radiation exposure. Inter. Congress Series., 1276: 181.
Hagen, V. (1989): Biochemical aspects of radiation biology. Experientia., 45:7.
Geraci, JP., and Mariano, MS. (1993):Radiation hepatology of the rat: Parenchymal and nonparenchymal cell injury. Rad. Res., 129: 322.
Levier, DG., Brown, RD., Mccay, TA., and Fuchs, BA.(1993): Hepatic and splenic phagocytosis in female mice implanted with morphine sulfate pellets. J. Pharmacol. Exp. Ther., 267(1):357-63.
Trigueiro, MS., General, F., Chossegros, S. (1992): Lack of hepatocyte involvement in the genesis of the sinusoidal dilatation related to heroin addiction a morphometric study. Virchows. Arch. Pathol. Anat. Histopathol., 420(2):149-153.
Curran, RC. (2000): Colour atlas of histology. Harvery miller publishers, Oxford University. Press. 4th ed.
Ahlersova, E., Ahlers, I., Slavkovska, M. (1981): Metabolic changes after non-lethal X-irradiation of rats. Folia. Biol., 27: 404.
El-Gharib, RF., Fahmy, N., Abd El Maguid, A., and Saber, FH. (1999): Studies on radiation injury and recovery on liver using silymarin. Egypt. J. Histol., 22(1-2):111.
AbdElMaguid, A., ElGharib, R., Ahmed, KA. (2004): Possible protectiveroleofmelatoninagainstradiationinducedhistopathologicaland histochemical changes in the liver of male albino rats. Egypt. J. Ger.Soc. Zool., 45C:1.
Radosevich, PM., Williams, PE., Brooks, LD., McRae, JR., Steiner, KE., and Cherrington, AD. (1984): Effect of morphine on glucose homeostasis in the conscious dog. J. Clin. Invest., 74:1473-1480.
Khamis, FI., Abu-Gabal,H.,Roushdy, HM. (1989): Control of radiation induced changes in certain serum and liver enzymes through bone marrow transplantation. 1st Internat. Conf. Low Cost Exp. Biophys. Cairo Univ.
Serizawa, N., Takei, Y., Okubo, H., Yamasina, S.,Enomoto, N., and Sato, N. (2006): Effect of low molecular weight heparin on the commitment of bone marrow cells to liver sinusoidal endothelial cells in CC14-induced liver injury. Hepatol. Res., 34(4): 207–213.
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