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Influence of Olive Leaf Extract and Stem Cell Therapy on Hepatic Cellular Injury Induced by Gamma Radiation in Albino Rats: Histological, Histochemical and Immunohistochemical Study
Current Issue
Volume 3, 2016
Issue 4 (July)
Pages: 63-73   |   Vol. 3, No. 4, July 2016   |   Follow on         
Paper in PDF Downloads: 52   Since Jul. 1, 2016 Views: 803   Since Jul. 1, 2016
Authors
[1]
Tamer M. M. Abu-Amara, Histology and Cytology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
[2]
Abd EL Razek A. Meselhy, Anatomy and Embryology Department, Faculty of Medicine, Al-Azhar University, Assiut, Egypt.
Abstract
Background: Free radicals are harmful molecules produced when our body breaks down foodparticles or via environmental factors like exposures to radiation. Free radicals can damage cells. Antioxidants such as olive leaf extract are substances that may protect our bodies against the effects of free radicals. Also, stem cell therapy is a new trend that can be used to treat these damaging effects. Aim of the work: The goal of this work is to study the radio-protective effect of olive leaf extract as a natural antioxidant and stem cell as a regenerative therapy on the liver of adult albino rats against the gamma radiation damaging effects. Material and Methods: The present study was carried out on 24 adult albino rats of local strain weighing 120±3 g which were randomly divided equally into Group 1(C, untreated negative control):the rats served as negative control (without any treatment for one month). Group 2 (I, Irradiated): The rats irradiated via exposing to 6 Gy delivered as a fractionated dose (2 Gy each 3 days). Group 3 (I+O, Irradiated+Olive leaf extract treatment): The rats had a combination of irradiation (in the same way like group 2) plus oral intake of olive leaf extract for one month. Group 4 (I+S, Irradiated+Stem cell therapy): The rats irradiated (in the same way like group 2) after single injection of mesenchymal stem cells (MSCs). Paraffin sections were prepared for histological, histochemical and immnuohistochemical studies. The data were statistically analysed. Results: Examined sections showed significant cellular injury in group 2 in comparison to the control group. Groups 3 and 4 showed obvious decrease in the pathological changes occurring in comparison with group 2. Conclusion: These results provide evidence that both olive leaf extract and stem cell therapy have radio-protective effect as they reduced the pathological cellular injuries in the liver induced by accumulated doses of radiation exposure. Further studies may be done to confirm these findings.
Keywords
Radiation, Liver, Olive, Stem Cells, Regenerative Medicine and Histochemsitry
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