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Acute Toxicity of Seed Aqueous Extract of Citrullus colocynthis to Fingerlings of the Nile Tilapia Oreochromis niloticus
Current Issue
Volume 2, 2015
Issue 5 (September)
Pages: 159-164   |   Vol. 2, No. 5, September 2015   |   Follow on         
Paper in PDF Downloads: 41   Since Sep. 19, 2015 Views: 1105   Since Sep. 19, 2015
Authors
[1]
Abdel Raheam H. A. El-Bassir, Department of Zoology, Faculty of Science and Technology, Omdurman Islamic University, Khartoum, Sudan.
[2]
Dina Ibrahim Mohamed Alim, Department of Natural resources, Environment and Natural Resources Research Institute, National Centre for Research, Khartoum, Sudan.
[3]
Mohamed Omer Ali, Department of Zoology, Faculty of Science and Technology, Omdurman Islamic University, Khartoum, Sudan.
[4]
Mohamed Ahmed Fath El - Rahman, Department of Zoology, Faculty of Science and Technology, Omdurman Islamic University, Khartoum, Sudan.
[5]
Ahmed Mohamed Zain, Department of Zoology, Faculty of Science and Technology, Omdurman Islamic University, Khartoum, Sudan.
Abstract
This study aimed at determining the acute toxicity of the aqueous extract of the seeds of Hanzal Citrullus colocynthis (CCT) to fingerlings of the Nile Tilapia Oreochromis niloticus (O. niloticus) . Six graded concentrations of the aqueous extract were determined after a screening test for 48 hours. Concentrations of 4, 2, 1, 0.5, 0.25 and 0.125 g/l and a control 0 g/l were used in a renewable bioassay for 96 hours. At various concentrations and exposure periods intoxication symptoms has been recorded. These included excitation, jumping darting movements, odd swimming and immobility. The physiochemical namely pH, temperature and dissolved oxygen tested were within tolerable levels. The lethal concentration which kills 50 % of the population (LC50) after 24 .48, 72 and 96 hours were 1.58, 0.785, 0.56, 0.50 g/L respectively. The time taken for 50% of the population to die (LT50) were 0.53, 16.48, 45.7, 40.74, 120.2, and 194.98 hours at concentrations 4, 2, 1, 0.5, 0.25 and 0.125 g/l respectively. The minimum concentration harmful to Tilapia was computed as 25.2mg/l. It was concluded that Citrullus colocynthis aqueous extract is classified as botanical substance with low toxicity to Tilapia fingerlings. It was recommended that this extract should be used but with precautions near fish inhabiting areas.
Keywords
Citrullus colocynthis, Hanzal, Acute Toxicity, Oreochromis niloticus
Reference
[1]
Abdel-Hassan I.A., Abdel-Barry J.A., Mohammeda S.T (2000). The hypoglycaemic and antihyperglycaemic effect of Citrullus colocynthis fruit aqueous extract in normal and alloxan diabetic rabbits. Journal of Ethnopharmacology. 71: 325-30.
[2]
Adam S.E.I., Al-Yahya M.A., Al-Farhan A.H. (2001). Combined toxicity of Cassia senna and Citrullus colocynthis in rats. Veterinary and Human Toxicology. 43: 70-2.
[3]
Alim, D.I. (2008). Studies on Some Effects of the Pesticide Endosulfan on the Nile Tilapia Oreochromis niloticus. Ph.D. Thesis, University of Khartoum.
[4]
Alsnafi, A.E. (2015). Bioactive components and pharmacological effects of canna indica - An overview. International Journal of Pharmacology & Toxicology. 5 (2), 71-75
[5]
Arnold, E .M. (1989). Aspects of a zooplankton, phytoplankton and phosphorus system, Ecol. Modelling 5 Techno. 18: 67–71.
[6]
Ayotunde, E.O. and B.O. Ofem, (2008). Acute and chronic toxicity of pawpaw (Carica papaya) seed powder to adult Nile tilapia (Oreochromis niloticus Linne 1757). Afr. J. Biotech., 7(13): 2265-2274.
[7]
Ayuba, V.O. and P.C. Ofojekwu, (2002). Acute toxicity of the root of Jimson’s weed, Datura innoxia to the African catfish, Clarias gariepinus fingerlings. J. Aquat. Sci., 17(2): 131-133.
[8]
Azare, B.A., S.K. Okwute and S.L. Kela, (2007). Molluscicidal activity of crude water leaf extracts of Alternanthera sesselis on Bulinus (phy) globosus. Afr. J. Biotech., 6(4): 441-444.
[9]
Burrows, G. And Shaik, R. (2015). Comparative developmental anatomy of the taproot of the cucurbitaceous vines Citrullus colocynthis (perennial), Citrullus lanatus (annual) and Cucumis myriocarpus (annual). Australian Journal of Botany 62(7) 537-545.
[10]
Chen, J.C., Chiu. M.H., Nie, R.L., Cordell, G. A. and Qiu S.(2005). Cucurbitacins and cucurbitane glycosides: structures and biological activities. Nat. Prod. Rep.; 22:386-399.
[11]
Diwan F.H, Abdel-Hassan I.A. and Mohammed S.T. (2000). Effect of Saponin on mortality and histopathological changes in mice. Eastern Me-diterranean Health Journal. 2000; 6 (2-3): 345-351.
[12]
Edriss, A.E.; Satti, A.A. and Alabjar, Z.A. (2013). Larvicidal properties of two asclepiadaceous plant species against the mosquito Anopheles arabiensis Patton (Diptera: Culicidae). J of Saudi Argi. Sci. 12(1): 59-66.
[13]
Lloyd, R. (1979). An Outline Scheme for evaluating the hazards to aquatic life from chemical pollution and obtaining water quality criteria. 6th FAO/SIDA, workshop on aqutic pollution in relation to protection of living resources. Mobassa – Kenya. FAO Rome T.F – RAF 112, pp: 154-165.
[14]
Maikai, V.A., P.I. Kobo and A.O. Adaudi, (2008). Acute toxicity studies of aqueous stem bark extract of Ximenia Americana. Afr. J. Biotech., 7(10): 1600-1603.
[15]
Miresmailli, S and Isman, M.B. (2014). Botanical insecticides inspired by plant–herbivore chemical interactions. Trends in plant Science. 19(1): 29-35.
[16]
OECD (1992). Report on the workshop on the extrapolation of laboratory aquatic toxicity data to the real environment. OECD, Paris, OECD Environment Monographs no. 59. OCDE/GD: 169, 43P.
[17]
Olufayo MO (2009). Haematological Characteristics of C. gariepinus juveniles exposed to Derris root powder. Afr. J. Food Agric. Nutr Dev. 9(3): 921 933.
[18]
Samara, F. (2001). Toxicological tests of pesticides. In A Training Course on Analysis of pesticides and their residues. Arab Organization for Agricultural Development. pp 126-142.
[19]
Sambasivam, S.; Karpagam, G.; Chandran, R. and Khan, S.A. (2003). Toxicity of leaf extract of yellow olcander Thevetia nerifolia on Tilapia. J.Environ.Biol.24:201-204.
[20]
Shaalan, E. A.; Canyon, D.; Younes, M.W.; Abdei-Wahab, H. and Mansour, A. (2005). A review of botanical phytochemicals with mosquitocidal potential. Environment nternational. 31(8): 1149-1166.
[21]
Soufane S.; Bedda A; Mahdeb Nand. Bouzidi A. (2013). Acute Toxicity study on Citrullus colocynthis fruit methanol extract in Albino rats .J. of App. Pharma. Sci. Vol. 3 (06), pp. 088-093.
[22]
Van den Heuvel, M.R., Power, M., Richards, J., Machinnon, M. and Dixon, D.G. (2000). Disease and gill lesions in yellow perch (perca flavescens) exposed to oil sands minig – associated waters. Ecotoxicology and Environmental safety, (46): 334. 341.
[23]
Walker, C. H.; Hopkin, S. P.; Sibly, R. M. and Peakall, D. B. (2001). Principles of Esctotoxicology. Taylor and Francis, London.
[24]
Yoshikawa, M., Morikawa, T., Kobayashi, H., Nakamura, A., Matsuhira, K., Nakamura, S. and Matsuda, H.(2007). Bioactive saponins and glycosides. XXVII. Structures of new cucurbitane-type triterpene glycosides and antiallergic constituents from Citrullus colocynthis. Chem. Pharm. Bull. 55:428-434.
[25]
Yumnamcha, T.; Nongthomba, U. and Damayanti Devi, M. (2014). Phytochemical screening and evaluation of genotoxicity and acute toxicity of aqueous extract of Croton tiglium. Intern. l J. of Sci. and Res. Publications. 4(1):1-5.
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