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Isolation and Identification of Aspergillus Species from Wooden Fish (Keumamah)
Current Issue
Volume 5, 2018
Issue 3 (September)
Pages: 27-35   |   Vol. 5, No. 3, September 2018   |   Follow on         
Paper in PDF Downloads: 41   Since Jul. 2, 2018 Views: 1109   Since Jul. 2, 2018
Authors
[1]
Resmila Dewi, Faculty of Biology, University of Gadjah Mada, Yogyakarta, Indonesia.
Abstract
Wooden fish (keumamah) is a traditional food made from tuna (Euthynnus affinis) coated with wheat flour and be drained. During the storage and marketing, wooden fish may be contaminated by certain types of fungi, especially Aspergillus. The presence of fungi contaminants would reduce the wooden fish quality and dangerous for consumption. This study aims were to isolate and identify Aspergillus species in wooden fish. The study commenced with analysis of fungal population contaminating wooden fish samples from five major markets in Banda Aceh and isolated through serial dilution and spread plate method. The growths were identified using standard mycological techniques. Based on the analysis the fungal population on wooden fish was 0.5x102 –3.5x102 CFU/g. Colonies the grew were then taken to the purified to obtain pure cultures. The result of identification obtained four Aspergillus species contaminants on wooden fish namely A. flavus (JIK-1), A. fumigatus (JIK-2), A. ochracheus (JIK-3), and A. niger (JIK-4). The results showed that samples of wooden fish examined, all were contaminated with A. flavus (100%).
Keywords
Isolation, Identification, Fungi, Aspergillus, Wooden Fish, Keumamah
Reference
[1]
Djarijal, A. S. Teknologi Tepat Guna Ikan Asin. Kanisius, Yogyakarta, 1995.
[2]
Moeljanto. Pengawetan dan Pengolahan Hasil Perikanan. Penebar Swadaya, Jakarta, 1992.
[3]
Fardiaz. Mikrobilogi Pangan. Gramedia Pustaka Utama, Jakarta, 1992.
[4]
Pal, M. Morbidity and mortality due to fungal infections. J. App. Microbiology and Biochemistry, 2017; 1 (1): 1-3.
[5]
Mousavi, B., Hedayati, M. T., Hedayati, N., Ilkit, M., and Syedmousavi, S. A review: Aspergillus species in indoor environments and their possible occupational and public health hazards. Curr Med Mycol, 2016; 2 (1); 36-42.
[6]
Hassane, A. M. A., El-Shanawany, A. A., Abo-Dahab, N. F., Abdel-Hadi, A. M., Abdul-Raouf, U. M., and Mwanza, M. Influence of Different Moisture Contents and temperature on growth and production of aflatoxin B1 by a toxigenic Aspergillus flavus isolated wheat flour. J. Eco. Heal Env, 2017; 5 (3): 77-83.
[7]
Samson, R. A., Visagie, C. M., Houbraken, J., Hong, S. B., Hubka, V., Klaassen, C. H. W., Perrone, G., Seifert, K. A., Susca, a., tanney, J. B., varga, J., Koscube, S., Szigeti, G., Yaguchi, T., and Frisvad, J. C. Philogeny, identification and nomenclature of the genus Aspergillus. Study in Mycology, 2014; 78: 141-173.
[8]
El Abed, N., Ibtissem, B. S., Mohamed, B. K., Mahmoud., M. H., and Naima B. M. Isolation and identification of fungal communities in organic and conventional soils. Int. J. Curr. Microbiol. App. Sci, 2017; 6 (4): 1111-1123.
[9]
Thathana, M. G., Murage, H., Abia, A. L. K., and Michael, P. Morphological characterization and determination of aflatoxin-production potentials of Aspergillus flavus isolated from maize and soil in Kenya. Agriculture, 2017; 7 (80): 1-14.
[10]
Nyongesa, B. W., Vincent, A., and Okoth. Identification key for Aspergillus species isolated from maize and soil of Nandi Country, Kenya. Advances in Microbiology, 2015; 5 (4): 205-229.
[11]
Srinivasan, T. and Saranraj, P. Isolation and identification of spoilage causing microorganisms in an Indian mackerel fish (Rastrelliger kanagurta). Int. J. Adv. Res. Biol. Sci, 2017; 4 (7): 1-7.
[12]
Alshannaq, A. and Jae-Hyuk, Y. A review: Occurrence, toxicity, and analysis of major mycotoxins in food. Int. J. Environ. Res. Public Health, 2017; 14: 1-20.
[13]
Aristil, J., Giovanni, V., and Alberto, S. Occurrence of toxigenic fungi and aflatoxin potential of Aspergillus spp. strains associated with subsistence formed crops in Haiti. J. Food Protections, 2017; 80 (4): 626-631.
[14]
Varga, J., Nikolett, B., Muthusamy, C., Csaba, V., and Sandor, K. Mycotoxin producers in Aspergillus genus; an update. Acta Biol Szaged, 2015; 59 (2): 151-167.
[15]
Alexopoulus, C. J., Mims, W., and Blackwell. Introductory Mycology. John Wile y & Sons, New York, 1996.
[16]
Samson, R. A., Hoekstra, E. S., Frisvad, J. C., and Filtenborg, O. Introduction to Food Borne Fungi, Ponsen & Looyen, Netherlands, 1999.
[17]
Pitt, J. I. and Hocking, A. D. Fungi and Food Spoilage, Second edition. Blackie Academic & Proffesional, London, 1997.
[18]
Barnett, H. L. and Hunter. Illustrated Genera of Imperfect Fungi. Burgess Publishing Company, 1998.
[19]
Watanabe, T. Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to species, Second Edition. CRC Press, New York, 2002.
[20]
Buckle, K. A., Edwards, R. A., Fleet, G. H., andWootton, M. IlmuPangan. UI-Press, Jakarta, 2013.
[21]
Akise, O. G., Abolagba, O. J., and Eyong, M. M. Mycoflora of tree fish species smoke-dried using rubber wood (HeveaBrassilensis) in Nigeria. Greener Journal of Agricultural Sciences 2013; 3 (5): 396-402.
[22]
Sani, F. M., Idris, A. N., and Gloria, T. Mycological evaluation of smoked-dried fish at Maiduguri metropolis Nigeria: preliminary findings and potential health implications. Appli Micro Open Access, 2016; 2 (1).
[23]
Ayotunde, E. O., Ada, F. B., Udeh, G. N., and Out, V. O. Weevils and fungi infestation of dried fish: A case study of Uyo local government area, Akwa Ibom state, Nigeria. Int. J. Fisheries and Aquatic Studies, 2016; 4 (4): 359-367.
[24]
Job, M. O., agina, S. e., and Dapiya, H. S. Occurrence of aflatoxigenic fungi in smoke-dried fish sold in Jos Metropolis. British Microbiology Research Journal, 2016; 11 (1): 1-7.
[25]
Mohamed, H. M. A., Walaa, F. A. E., Albert, B., and Seddik, H. Detection of aflatoxin-producing fungi isolated from Nile tilapia and fish feed. EXCLI Journal, 2017; 16: 1308-1318.
[26]
Salawudeen, M. T., Kazeem, H. M., Raji, M. A., Oniye, S. J., Kwanashie, C. N., and Ibrahim, M. J. Isolation and identification of fungi from apparently healty and diseased Clarias gariepinus from freshwater in Zaria, Kaduna State, Nigeria. Microbiol Res, 2017; 5 (1): 8-15.
[27]
Abbas, M. S., Jenan, M. K., and Shaimaa, N. Y. Isolation and identification of systemic mycological isolates from fishes samples that obtained from local markets in Baghdad, Iraq. World J. Exp. Biosci, 2016; 4 (2): 143-146.
[28]
Olajuyigbe, O., Esther, A., and Kafayat, Y. Occuurence of fungi species in some fisheries product from retail markets in Lagos, Nigeria. Food and Applied Biocience, 2017; 5 (3): 149-154.
[29]
Emmanuel, B. E. and Hontoyon, E. W. Microbiological quality of cold smoked sardine, Sardinella maderensis in four markets in Lagos Metropolis, Lagos State, Nigeria. Journal of Aquatic Science, 2016; 31 (1): 167–184.
[30]
Adebayo-Tayo, B. C., Onilude, A. A. and Patrick, U. C. Mycofloral of Smoke-Dried Fishes Sold in Uyo, Eastern Nigeria. World Journal of Agricultural Sciences, 2008; 4 (3): 346–350.
[31]
Belitz, H. D. and Grosch, W. 2010. Food Chemistry. Springer, Berlin, 2010.
[32]
Fitasari, E. Pengaruh tingkat penambahan tepung terigu terhadap kadar air, kadar lemak, kadar protein, mikrostruktur dan mutu organolepttik keju gouda olahan. Jurnal Ilmu dan Teknologi Hasil Ternak, 2009; 2 (2): 17-29.
[33]
Eaton, D. L. and Groopman, J. D. 1994. The Toxicology of Aflatoxins. Academic Press, New York, 1999.
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