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Correlation Between HPLC Identification Technique of Rocket as Antifungal
Current Issue
Volume 5, 2018
Issue 3 (September)
Pages: 36-45   |   Vol. 5, No. 3, September 2018   |   Follow on         
Paper in PDF Downloads: 18   Since Jul. 5, 2018 Views: 162   Since Jul. 5, 2018
Authors
[1]
Husain Abd Allah El-Fadaly, Agriculture Microbiology Department, Faculty of Agriculture, Damietta University, Damietta City, Egypt.
[2]
Sherif Mohamed El-Kadi, Agriculture Microbiology Department, Faculty of Agriculture, Damietta University, Damietta City, Egypt.
[3]
Mostafa Maher El-Moghazy, Animal Production Department, Faculty of Agriculture, Damietta University, Damietta City, Egypt.
[4]
Ahmed Ali Soliman, Animal Nutrition Research Department, Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Giza City, Egypt.
[5]
Mahmoud Salama Mahmoud El-Haysha, Animal Nutrition Research Department, Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Giza City, Egypt.
Abstract
Eruca sativa is an edible annual plant, commonly known as rocket salad or arugula. In this paper, the polyphenolic compounds of 3 extracts of E. sativa meal and oil have been investigated. The analytical procedures revealed Twenty-five polyphenolic and Eleven flavonoids compounds by RP-HPLC Chromatograms obtained for extracts. The crude extracts and the 36 purified compounds were tested against fungi Helminthosporium solani, Botrytis cinerea, Botrytis fabae, Fusarium oxysporum, and Fusarium solani. Methanol, Hexane, Heat treatment-methanol extracts, and oil were added to Potato Dextrose Agar (PDA) at concentrations of 1, 2, and 4 mg/ml. Respective inhibited the mycelial growth, monogenesis/sporulation, and spore germination of both tested fungi. The environmental studies of chemistry, biochemistry, and pharmacological characteristic of something of flavonoids and polyphenolic may be of great significance. For that reason, it is stimulating to investigate a relationship between the biosynthetic origin of the antifungal compound and its mode of action against fungi. The present study was designed to screen out E. sativa extracts on fungal pathogens and increase the shelf life of products. Information gathered from this study provides an important basis for further study into the uses of E. sativa compounds for control of postharvest diseases of potato and anther vegetable's, Albeit partially. As natural compounds produced from a small remaining quantity of something of the watercress seeds industry to preserve the environment and crops, and thus it became the residue economic and practical value.
Keywords
Eruca sativa, Extracts, Oil, HPLC, Antifungal, Secondary Metabolites, Antibiotic Alternatives, Animal/Plant Diseases
Reference
[1]
Vig, A. P.; Walia, A. (2001). Beneficial effects of Rhizopus oligo sporous fermentation on reduction of glucosinolates, fiber, and phytic acid in rapeseed (Brassica napus) meal. Bioresource Technol., 78, 309-312.
[2]
Rakariyatham, N.; Sakorn, P. (2002). Biodegradation of glucosinolates in brown mustard meal (Brassica juncea) by Aspergillus sp. NR-4201 in liquid and solid culture. Biodegradation 3: 395-409.
[3]
Talalay, P.; Fahey, J. W. (2001). Phytochemicals from cruciferous plants protect against cancer by modulating carcinogen metabolism. J. Nutr., 131: 3027-33.
[4]
Bradley, P. R. (1992). British herbal medicine association. British herbal compendium, vol. 1. Rolnic. Zych, 434: 395-399.
[5]
Leung, A. Y.; Foster, S. (1996). Drugs and cosmetics, 2nd Encyclopedia of Common Natural ingredients used in food New York: john wiley & sons, Inc.
[6]
Kim, S. J.; Jin, S.; Ishii, G. (2004). Isolation and structural elucidation of 4-(B-d-lucopyranosyldisulfanyl) butyl glucosinolate from leaves of rocket salad (Eruca sativa L) and its antioxidative activity Biosci. Biotechnol. 68: 2444-2450.
[7]
Chen, S.; Andreasson, E. (2001). Update on glucosinolate metabolism and transport. Plant Physiol. Biochem. 39, 743-758.
[8]
Kutz, A.; Muller, A.; Hennig, P.; Kaiser, W. M.; Piotrowski, M.; Weiler, E. W. (2002). A role for nitrilase 3 in the regulation of root morphology in sulphur-starving Arabidopsis thaliana. Plant J. 30, 95-106.
[9]
Grubb, C. D.; Zipp, B. J.; Muller, J. L.; Masuno, M. N.; Molinski, T. F.; Abel, S. (2004). Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis. Plant. J. 40, 893-908.
[10]
Chan, M. K. Y.; Close, R. C. (1987). Aphanomyces root rot of peas control by the use of cruciferous amendments. New Zealand Journal of Agricultural Research, 30, 225-233.
[11]
Vierheilig, H.; Ocampo, J. A. (1990). Effect of isothiocyanates on germination of spores of G. mosseae. Soil Biology and Biochemistry, 22, 1161-1163.
[12]
Gamliel, A.; Stapleton, J. J. (1993). Characterization of antifungal volatile compounds evolved from solarized soil amended with cabbage residues. Phytopathology, 83: 899-905.
[13]
Angus, J. F. (1994). Biofumigation: isothiocyanates released from Brassica roots inhibit the growth of the take-all fungus. Plant and Soil, 162: 107-112.
[14]
Samuelsson, G.; Bohlin, L. (2009). Drugs of Natural Origin. (6th edn) Apotekarsocieteten, Sweden.
[15]
Agostini-Costa. T. S.; VRF, Bizzo, H. R.; Silveira, D.; Gimenes, M. A. (2012). Secondary Metabolites. In: Chromatography and its applications. Dhanarasu DS (ed) In Tech Brazil.
[16]
Rani, I.; Akhund, S.; Suhail, M.; Abro, H. (2010). Antimicrobial potential of seed extract of Eruca sativa. Pak J Bot, 42 (4), 2949-2953.‏
[17]
Abdel-Rahman, S., Haggag, A., & Elmaghraby, A. (2015). Activation of PTEN tumor suppressor gene expression by Eruca sativa seeds extract against rat mammary gland carcinogenesis induced by DMBA. Australian Journal of Basic and Applied Sciences, 9 (23), 431-6.‏
[18]
Matusheski, N.; Jeffery, E. H. (2001). Comparison of the bioactivity of two glucoraphanin hydrolysis products found in broccoli, sulforaphane and sulforaphane nitrile. J. Agric. Food Chem. 49: 5743-5749.
[19]
Jen, J.; Lin, T.; Huang, J.; Chung, W. (2001). Direct determination of sinigrin in mustard seed without desulfatation by reverse-phase ion-pair liquid chromatography. J. Chrom. A 912: 363-368.
[20]
Verkerk, R.; van Boekel, M. A. J. S.; Jongen, W. M. F.; Dekker, M. (2002). Predictive modeling of the glucosinolate myrosinase system during cooking of cabbage. In: Verkerk R. (Eds.) Evaluation of glucosinolate levels throughout the production chain of Brassica vegetables; towards a novel predictive modeling approach, Ph.D. Thesis, Wageningen University. pp 87110.
[21]
Tsao, G.; Yu, Q.; Potters, J.; Chiba, M. (2002). Direct and simultaneous analysis of sinigrin and allyl isothiocyanates in mustard samples by high-performance liquid chromatography. J. Agric. Food Chem. 50: 4749-4753.
[22]
Karcher, A.; Rassi, Z. E. (1999). Capillary electrophoresis of glucosinolates and their degradation products. Electrophoresis 20: 3181-3189.
[23]
Padukka, I.; Bhandari, B.; D’Arcy, B. (2000). Evaluation of various extraction methods of decomposition of encapsulated oil from β-cyclodextrin-melon oil complex powder. J. Food Comp. Anal. 13: 59-70.
[24]
Ben-Hammouda, M.; Kremer, R. J.; Minor, H. C.; Sarwar, M. (1995). A chemical basis for differential allelopathic potential of sorghum hybrids on wheat. J. Chem. Ecol. 21: 775-786.
[25]
Pintado, J.; González, M. P.; Murado, M. A. (1997). Interactions between pretreatment and nutrient concentrations of mussel processing for citric acid production. Enzyme and Microbial Technol., 20: 544-549.
[26]
Elsherbiny, A. E.; El-Khateeb, A. Y. (2012). Effect of organic and inorganic salts on mycelial growth, sporulation and spore germination of potato postharvest pathogens. J. Plant Prot. and Pathol., Mansoura Univ., 3 (12): 1353-1364.
[27]
Plethora of polyphenols Plant Phenolics (1989). Edited by Harborne, J. B. Volume 1 in Methods in Plant Biochemistry (Dey, P. M.; Harborne, J. B. Eds).
[28]
Hättenschwiler, S.; Vitousek, P. M. (2000). The role of polyphenols in terrestrial ecosystem nutrient cycling. Trends Ecol Evol. 15 (6): 238-243.
[29]
Tahara, S.; Ingham, J. L.; Nakahara, S.; Mizutani, J.; Harborne, J. B. (1984). Fungitoxic dihydro furan isoflavones and related compounds in white lupin, Lupinus albus. Phytochem., 23: 1889-1900.
[30]
Lago, J. H.; Ramos, C. S.; Casanova, D. C.; Morandim, A. A.; Bergamo, D. C.; Cavalheiro, A. J.; Bolzani, V. S.; Furlan, M.; Guimaraes, E. F.; Young, M. C.; Kato, M. J. (2004). Benzoic acid derivatives from Piper species and their fungitoxic activity against Cladosporium cladosporioides and C. sphaerospermum. J. Nat. Prod., 67: 783-792.
[31]
Sadiq, A.; Hayat, M. Q.; Mall, S. M. (2014). Qualitative and quantitative determination of secondary metabolites and antioxidant potential of Eruca sativa. Natural Products Chemistry & Research.‏
[32]
El-Fadaly, H. A.; El-Kadi, S. M.; El-Moghazy, M. M.; Soliman, A. A.; El-Haysha, M. S. M. (2017a). Correlation Between Active Components of Rocket (Eruca sativa) as Cytotoxicity (Brine Shrimp Lethality Assay). American Journal of Biomedical Science and Engineering. Vol. 3, No. 2, pp. 20-24.
[33]
El-Fadaly, H. A.; El-Kadi, S. M.; El-Moghazy, M. M.; Soliman, A. A. M.; El-Haysha, M. S. (2017b). Antioxidant activity studies on extracts of Eruca sativa seed meal and oil, detoxification, the role of antioxidants in the resistant microbes. IJSRM Human J., 6 (3): 31-51.
[34]
Abu-Zinadah, O. A. (2008). Effects of watercress oil on the thermal and chemical burn injuries in rabbits. Medical Science, Vol. 15 No. 4, pp.: 3-17.
[35]
Aqil, F.; Ahmad, I. (2003). Broad-spectrum antibacterial and antifungal properties of certain traditionally used Indian medicinal plants. World J. Microbiol. Biotechnol., 19: 653-657.
[36]
Stevenson, W. R.; Loria, R..; Franc, G. D.; Weingartner, D. P. (2001). Compendium of Potato diseases, 2nd P edition. APS Press, USA.
[37]
Walker, J. C.; Stahmann, M. A. (1955). Chemical Nature of Disease Resistance in Plants. Annu. Rev. Plant Physiol. 6: 351-366.
[38]
Lee, S.; LeTourneau, D. J. (1958). Chlorogenic acid content and Verticillium wilt resistance of potatoes. Phytopathol. 48: 268-274.
[39]
Wilson, C. L.; Wisniewski, M. E. (1989). Biological Control of Postharvest Diseases of Fruits and Vegetables: An Emerging Technology. Annu. Rev. Phytopathol. 27: 425-441.
[40]
Freiesleben, S, H,; Jäger, A. K. (2014). Correlation between Plant Secondary Metabolites and Their Antifungal Mechanisms–A Review. Med Aromat Plants. 3: 154. doi: 10.4172/2167-0412.1000154.
[41]
Ashour ML, Wink M (2011). Genus Bupleurum: A review of its phytochemistry, pharmacology, and modes of action. J Pharma Pharmacol. 63: 305-321.
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