Correlation Between HPLC Identification Technique of Rocket as Antifungal

Husain Abd Allah El-Fadaly; Sherif Mohamed El-Kadi; Mostafa Maher El-Moghazy; Ahmed Ali Soliman; Mahmoud Salama Mahmoud El-Haysha

doi:7080256

Eruca sativa is an edible annual plant, commonly known as rocket salad or arugula. In this paper, the polyphenolic compounds of 3 extracts of E. sativa meal and oil have been investigated. The analytical procedures revealed Twenty-five polyphenolic and Eleven flavonoids compounds by RP-HPLC Chromatograms obtained for extracts. The crude extracts and the 36 purified compounds were tested against fungi Helminthosporium solani, Botrytis cinerea, Botrytis fabae, Fusarium oxysporum, and Fusarium solani. Methanol, Hexane, Heat treatment-methanol extracts, and oil were added to Potato Dextrose Agar (PDA) at concentrations of 1, 2, and 4 mg/ml. Respective inhibited the mycelial growth, monogenesis/sporulation, and spore germination of both tested fungi. The environmental studies of chemistry, biochemistry, and pharmacological characteristic of something of flavonoids and polyphenolic may be of great significance. For that reason, it is stimulating to investigate a relationship between the biosynthetic origin of the antifungal compound and its mode of action against fungi. The present study was designed to screen out E. sativa extracts on fungal pathogens and increase the shelf life of products. Information gathered from this study provides an important basis for further study into the uses of E. sativa compounds for control of postharvest diseases of potato and anther vegetable's, Albeit partially. As natural compounds produced from a small remaining quantity of something of the watercress seeds industry to preserve the environment and crops, and thus it became the residue economic and practical value.

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