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Production of Siderophores in Oil Enriched Seawater Samples Collected at Different Depths
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Volume 2, 2015
Issue 3 (June)
Pages: 56-64   |   Vol. 2, No. 3, June 2015   |   Follow on         
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Francesca Crisafi, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Daniela Russo, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Santina Mangano, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Cinzia Pellicorio, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Santina Santisi, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Michail Yakimov, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Renata Denaro, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
The present study reports a selection of bacterial siderophore-producers in oil-enriched seawater samples collected along the water column, namely, surface, maximum chlorophyll and maximum depth in the north-western Mediterranean sea. Samples were enriched with oil and maintained in iron-limitation conditions for 20 days and monitored for siderophore production, growth performance and hydrocarbons degradation. Cultures showing firstly positive response at the liquid CAS assay were plated on CAS solid medium and the colonies showing orange halo were isolated and identified. Pure cultures of the isolates were then tested for the functional group of the produced siderophores. As results we obtained strains capable to produce siderophores in particular environment conditions: Marinobacter sp. and Alcanivorax sp., in surface water, Halomonas sp., and Marinobacter sp., at depth where maximum chlorophyll was detected and Alteromonas sp., Pseudoalteromonas sp., Vibrio sp. and Alcanivorax sp. in deep water. The surface and maximum chlorophyll depth enrichments showed the 80% of degradation while deep samples growing slowly degraded the 48% of hydrocarbons in the same time. The functional groups of siderophores were equally distributed in all the tested samples. In conclusion, we have identified the cultivable hydrocarbon-degrading bacteria which could function as helper-strains favoring the hydrocarbon-degradation processes in iron-stress conditions.
Hydrocarbons Degrading Bacteria, Siderophores, Iron, Water Column, Bioremediation
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