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Production of Siderophores in Oil Enriched Seawater Samples Collected at Different Depths
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Volume 2, 2015
Issue 3 (June)
Pages: 56-64   |   Vol. 2, No. 3, June 2015   |   Follow on         
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Francesca Crisafi, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Daniela Russo, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Santina Mangano, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Cinzia Pellicorio, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Santina Santisi, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Michail Yakimov, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Renata Denaro, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
The present study reports a selection of bacterial siderophore-producers in oil-enriched seawater samples collected along the water column, namely, surface, maximum chlorophyll and maximum depth in the north-western Mediterranean sea. Samples were enriched with oil and maintained in iron-limitation conditions for 20 days and monitored for siderophore production, growth performance and hydrocarbons degradation. Cultures showing firstly positive response at the liquid CAS assay were plated on CAS solid medium and the colonies showing orange halo were isolated and identified. Pure cultures of the isolates were then tested for the functional group of the produced siderophores. As results we obtained strains capable to produce siderophores in particular environment conditions: Marinobacter sp. and Alcanivorax sp., in surface water, Halomonas sp., and Marinobacter sp., at depth where maximum chlorophyll was detected and Alteromonas sp., Pseudoalteromonas sp., Vibrio sp. and Alcanivorax sp. in deep water. The surface and maximum chlorophyll depth enrichments showed the 80% of degradation while deep samples growing slowly degraded the 48% of hydrocarbons in the same time. The functional groups of siderophores were equally distributed in all the tested samples. In conclusion, we have identified the cultivable hydrocarbon-degrading bacteria which could function as helper-strains favoring the hydrocarbon-degradation processes in iron-stress conditions.
Hydrocarbons Degrading Bacteria, Siderophores, Iron, Water Column, Bioremediation
Yakimov MM, Timmis KN, Golyshin PN (2007). Obligate oil-degrading marine bacteria. Curr. Opin. Biotechnol. 18 (3), 257–266.
Wang Q, Zhang S, Li Y, Klassen W (2011). Potential Approaches to Improving Biodegradation of Hydrocarbons for Bioremediation of Crude Oil Pollution. J. Environ. Prot 2, 47-55.
Harayama S, Kishira H, Kasai Y, Shutsubo K (1999). Petroleum Biodegradation in Marine Environments. J. Molec. Microbiol. Biotechnol 1, 63-70.
Nilanjana D, Chandran P. Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview. Biotechnology Research International. Volume 2011, Article ID 941810, 13 pages
Denaro R, Crisafi F, Russo D, Genovese M, Messina E, Genovese L, Carbone M,. Ciavatta ML, Ferrer M, Golyshin P, Yakimov MM (2014). Alcanivorax borkumensis produces an extracellular siderophore in iron-limitation condition maintaining the hydrocarbon-degradation efficiency. Marine Genomics 17, 43–52.
Kem MP, Zane HK, Springer SD, Gauglitz JM, Butler A (2014). Amphiphilic siderophore production by oil-associating microbes. Metallomics. 6(6), 1150-5
Coale KH, Johnson KS, Fitzwater SE, Gordon RM, Tanner S, Chavez FP, Ferioli L, Sakamoto C, Rogers P, Millero F, Steinberg P, Nightingale P, Cooper D, Cochlan WP, Landry MR, Constantinou J, Rollwagen G, Trasvina A, Kudela R (1996). A massive phytoplankton bloom induced by an ecosystem-scale iron fertilization experiment in the equatorial Pacific Ocean. Nature. 383(6600):495 - 501.
Martin J, Raibaud A, Ollo R (1994). Testing the iron hypothesis in ecosystems of the equatorial Pacific Ocean. Nature 371, 123 - 129
Butler A (2005). Marine siderophores and microbial iron mobilization. Biometals. 18, 369–374.
Homann VV, Edwards KJ, Webb EA, Butler A (2009). Siderophores of Marinobacter aquaeolei: petrobactin and its sulfonated derivatives. Biometals 22 (4), 565–571.R.
Amin SA, Green DH, Hart MC, Küpper FC, Sunda WG, Carrano CJ (2009). Photolysis of iron-siderophore chelates promotes bacterial-algal mutualism. Proc Natl Acad Sci U S A. 6;106(40):17071-6. doi: 10.1073/pnas.0905512106.
Martinez JS, Zhang GP, Holt PD, Jung H-T, Carrano CJ, Haygood MG, Butler A (2000). Self-assembling amphiphilic siderophores from marine bacteria. Science. 287:1245–1247.
Martinez JS, Carter-Franklin JN, Mann EL, Martin JD, Haygood MG, Butler, A (2003). Structure and membrane affinity of a suite of amphiphilic siderophores produced by a marine bacterium. Proc. Natl. Acad. Sci. USA 100, 3754–3759.
Barbeau K, Rue EL, Trick CG, Bruland KW, Butler A. (2003) The photochemical reactivity of siderophores produced by marine heterotrophic bacteria and cyanobacteria based on characteristic iron(III)- binding groups. Limnol Oceanogr. 48, 1069–1078.
Macrellis HM, Trick CG, Rue EL, Smith G, Bruland KW (2001). Collection and detection of natural iron-binding ligands from seawater. Mar. Chem. 76: 175–187.
Fung I Y, Meyn S K, Tegen I, Doney S C, John J G, Bishop B. (2000). Iron supply demand in the upper ocean. GLOBAL BIOGEOCHEMICAL• CYCLES, VOL. 14, NO. 1, PAGES 281-295-
La Ferla R, Azzaro F, Azzaro M, Caruso G, Dicembrini F, Leonardi M, et al. (2005). Microbial contribution to carbon biogeochemistry in the Central Mediterranean Sea: variability of activities and biomass. J Mar Syst 57: 146–166.
Dyksterhouse SE, Gray JP, Herwig RP, Lara, JC, Staley, JT (1995). Cycloclasticus pugetii gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium from marine sediments. Int. J. Syst. Bacteriol. 45 (1), 116–123.
Porter KG, Feig YS (1980). The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25, 943–948
Troussellier M, Got P, Mboup M, Corbin D, Giuliano L et al (2005). Daily bacterioplankton dynamics in a sub-Saharan estuary (Senegal River, West Africa): a mesocosm study. Aquatic microbial ecology 40 (1), 13-24
Winnepenninckx B, Backeljau T, De Wachter R (1993). Extraction of high molecular weight DNA from molluscs. Trends in Genetics 9: 407.
Lane DJ (1991) 16/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics ed. Stackebrandt, E. and Goodfellow, M. 115–175.
Maidak BL, Olsen GJ, Larsen N, et al (1997). The RDP (Ribosomal Database Project). Nucleic Acids Research 25(109): 1-11.
Pearson W, Lipman D (1988) Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. U.S.A. 85: 2444–2448
Cappello S, Russo D, Santisi S, Calogero R, Gertler C, Crisafi F. et al. (2012) Presence of hydrocarbon-degrading bacteria in the gills of mussel Mytilus galloprovincialis in a contaminated environment: a mesoscale simulation study. Chemistry and Ecology 28 (3): 239-252
Yakimov MM, Cappello S, Crisafi E, Tursi A, Corselli C, Scarfı`S, Giuliano L (2006). Phylogenetic survey of metabolically active microbial communities associated with the deep-sea coral Lophelia pertusa from the Apulian Plateau, Central Mediterranean Sea. Deep Sea Res. Part I 53: 62–75.
Schwyn B, Neilands JB (1987). Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160 (1), 47–56.
Arnow L E. (1937) Colorimetric determination of the components of 3,4 dihydroxyphenylalanine-tyrosine mixtures. J. Biol. Chem. 118:531-537.
Young G, Cox G B, Cox, Gibson F (1967) 2,3-Dihydroxybenzoate as a bacterial growth factor and its route of biosynthesis. Biochim. Biophys. Acta 141:319-331.
Emery T, and Neilands J B. (1960) Periodate oxidation of hydroxylamine derivatives. Products, scope, and application. J. Am. Chem. Soc. 82:4903-4904.
Emery, T F, Neilands J B (1962) Further observations concerning the periodic acid oxidation of hydroxylamine derivatives. J. Org. Chem. 27:1075-1077.
Denaro R, D’Auria G, Di Marco G, Genovese M, Troussellier M, Yakimov MM, Giuliano L (2005) Assessing terminal restriction fragment length polymorphism suitability for the description of bacterial community structure and dynamics in hydrocarbon-polluted marine environments. Environmental Microbiology 7: 78–87.
Bouchez M, Blanchet D, Vandecasteele JP (1995). Degradation of polycyclic aromatic hydrocarbons by pure strains and by defined strain association: inhibitions phenomena and comate-bolism. Appl Environ Microbiol 43, 156-164.
Amin S A, Parker M S, Armbrust E V. (2012). Interactions between diatoms and bacteria. Microbiology and Molecular Biology Reviews, 76(3), 667-684.
Kanoh K, Kamino K, Leleo G, Adachi K, Shizuri Y. (2003). Pseudoalterobactin A and B, new siderophores excreted by marine bacterium Pseudoalteromonas sp. KP20-4. The Journal of antibiotics, 56(10), 871-875.
Zane H K, Butler A. (2013). Isolation, structure elucidation, and iron-binding properties of lystabactins, siderophores isolated from a marine Pseudoalteromonas sp. Journal of natural products, 76(4), 648-654.
Reid G, Sharma S, Advikolanu K, Tieszer C, Martin RA, Bruce A W. (1994). Effects of ciprofloxacin, norfloxacin, and ofloxacin on in vitro adhesion and survival of Pseudomonas aeruginosa AK1 on urinary catheters. Antimicrobial agents and chemotherapy, 38(7), 1490-1495.
Holt H M, Gahrn‐Hansen B, Bruun B. (2005). Shewanella algae and Shewanella putrefaciens: clinical and microbiological characteristics. Clinical microbiology and infection, 11(5), 347-352.
Actis L A, Fish W, Crosa J H, Kellerman K, Ellenberger SR, Hauser F M, Sanders-Loehr J. (1986). Characterization of anguibactin, a novel siderophore from Vibrio anguillarum 775 (pJM1). Journal of bacteriology, 167(1), 57-65.
Zane H K, Naka H, Rosconi F, Sandy M, Haygood M G, Butler A. (2014). Biosynthesis of Amphi-enterobactin Siderophores by Vibrio harveyi BAA-1116: Identification of a Bifunctional Nonribosomal Peptide Synthetase Condensation Domain. Journal of the American Chemical Society, 136(15), 5615-5618.
Heller MI, Gaiero DM, Croot PL (2013). Basin scale survey of marine humic fluorescence in the Atlantic: Relationship to iron solubility and H2O2. Global Biogeochemical Cycles 27, 88–100.
Sheppard PJ, Keryn LS, Krishna KK, Sayali SP, Andrew S. Ball (2012). The Importance of Weathered Crude Oil as a Source of Hydrocarbonoclastic Microorganisms in Contaminated Seawater J. Microbiol. Biotechnol. 22(9), 1185–1192
Cappello S, Denaro R, Genovese M, Giuliano L, Yakimov M (2007). Predominant growth of Alcanivorax during experiments on "oil spill bioremediation" in mesocosms. Microbiol Res. 162(2):185-90
Gutierrez T, Singleton DR, Berry D, Yang T, Aitken MD, Teske A (2013). Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP. ISME J 7: 2091–2104.
Genovese M, Crisafi F, Denaro R, Cappello S, Russo D, Calogero R, Santisi S, Catalfamo M, Modica A, Smedile F, Genovese L, Golyshin PN, Giuliano L, Yakimov MM (2014). Effective bioremediation strategy for rapid in situ cleanup of anoxic marine sediments in mesocosm oil spill simulation. Front. Microbiol. 5:162.
McKew BA, Coulon F, Yakimov MM, Denaro R, Genovese M, Smith CJ, Osborn AM, Timmis KN, McGenity TJ (2007). Efficacy of intervention strategies for bioremediation of crude oil in marine systems and effects on indigenous hydrocarbonoclastic bacteria. Environm. Microbiol. 9(6): 1562-1571.
Cappello S, Genovese M, Denaro R, Santisi S, Volta A, Bonsignore M, Mancini G, Giuliano L, Genovese L, Yakimov M (2014). Quick stimulation of Alcanivorax sp. by bioemulsificant EPS2003 on microcosm oil spill simulation. Braz J Microbiol. 45(4): 1317–1323.
Jin HM, Kim JM, Lee HJ, Madsen EL, Jeon CO (2012). Alteromonas as a key agent of polycyclic aromatic hydrocarbon biodegradation in crude oil-contaminated coastal sediment. Environ Sci Technol. 17;46(14):7731-40
Amin SA (2010). The role of siderophores in algal-bacterial interactions in the marine environment. UC San Diego: b6880783.
Lazzari P, Solidoro C, Ibello V, Salon S, Teruzzi A, Béranger K, Crise A (2012). Seasonal and inter-annual variability of plankton chlorophyll and primary production in the Mediterranean Sea: a modelling approach. Biogeosciences, 9(1), 217-233.
Gutierrez T, Biller DV, Shimmield T, Green DH (2012). Metal binding properties of the EPS produced by Halomonas sp. TG39 and its potential in enhancing trace element bioavailability to eukaryotic phytoplankton. Biometals, 25(6), 1185-1194.
Keshtacher-Liebso E, Hadar Y, Chen Y (1995). Oligotrophic bacteria enhance algal growth under iron-deficient conditions. Appl Env Microbiol 61, 2439–2441.
Yakimov M M, La Cono V, Smedile F, Crisafi F, Arcadi E, Leonardi M, ... & Giuliano L (2014). Heterotrophic bicarbonate assimilation is the main process of de novo organic carbon synthesis in hadal zone of the Hellenic Trench, the deepest part of Mediterranean Sea. Environmental Microbiology Reports, 6(6), 709-722.
Tapilatu Y, Acquaviva M, Guigue C, Miralles G, Bertrand JC Cuny P (2010). Isolation of alkane-degrading bacteria from deep-sea Mediterranean sediments. Letters in Applied Microbiology 50, 234–236.
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