Isolation and Characterization of Oil-Degrading Bacteria from Bilge Water
[1]
Santina Santisi, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy; Dep. Environmental and Biological Sciences, Faculty of MM.FF.NN, Ph.D School in “Biology and Cellular Biotechnology”, University of Messina, Messina, Italy.
[2]
Gabriella Gentile, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
[3]
Anna Volta, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy; Dep. of Industrial and Mechanical Engineering, Faculty of Engineering, University of Catania, Catania, Italy.
[4]
Martina Bonsignore, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
[5]
Giuseppe Mancini, Dep. of Industrial and Mechanical Engineering, Faculty of Engineering, University of Catania, Catania, Italy.
[6]
Paola Quatrini, Department STEBICEF, Faculty of MM. FF. NN, University of Palermo, Palermo, Italy.
[7]
Simone Cappello, Institute for Coastal Marine Environment (IAMC) – CNR of Messina, Messina, Italy.
Twenty-one oil-degrading bacteria were isolated from bilge water. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 7 strains were selected (from 21 isolated) for further studies. 16S rRNA gene sequencing showed that isolated strains were affiliated to Bacillus, Pseudomonas and Halomonas genera; in particular, isolate BW-B12 (Bacillus sp., 99%), BW-C12 (Halomonas boliviensis, 99%) and BW-E12 (Halomonas boliviensis, 98%) were the best crude-oil degraders; after 10 days of cultivation in ONR 7a mineral medium supplemented with crude oil as single carbon source BW-B12, BW-C12 and BW-E12 showed a degradation rate of 80, 60 and 59%, respectively. The strains showed also a high emulsification activity and biosurfactants production. Obtained results give an important contribution in order to utilize these bilge water autochthonous microorganisms in processes of bioremediation of marine environment chronically polluted from saline oily waste.
Bilge Water, Bioremediation, Marine Pollution, Oil Degrading Bacteria, Saline Oily Waste
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