Rapid Diagnosis of Acute Respiratory Infections by Multiplex Endpoint PCR Technology
[1]
Aurelian Udristioiu, Clinical Laboratory Department, Emergency County Hospital Targu Jiu & UCB University, City Targu Jiu, Romania.
[2]
Manole Cojocaru, Physiology Department, Faculty of Medicine, Titu Maiorescu University, City Bucharest, Romania.
[3]
Dana Alexandra Maria Panait, Microbiology Department, Faculty of Medicine, Titu Maiorescu University, City Bucharest, Romania.
[4]
Nica Badea Delia, Biochemistry Department, Faculty of Medical Science, Behavioral Constantin Brancusi University, City Targu Jiu, Romania.
Introduction: The multiplex endpoint PCR technology offers a number of potential advantages, results are available in a matter of hours rather than days, the extreme sensibility facilitates detection of even minutes the amounts of pathogen DNA in clinical samples and the test is not significantly affected by prior administration of antibiotics. Aim: The aim of this work was to rapidly identify the antibiotic resistance the monitoring of pathogen growth at the patients admitted in Hospitalization Intensive Care Unit of Emergency County Hospital Targu Jiu with the diagnosis of Community Acquired Pneumonia, (CAP), in months December/2013-March/2014. Method: The Analyzer Unyvero™ Pneumonia Application was used in detection of pneumonia associated pathogens and their antibiotic resistance genes using the Pneumonia Unyvero™ System, following PCR pathogen species with sequencing of the amplified microbial DNA. Results: The main pathogens of community acquired pneumonia from the cohort study, 36 cases, (20 males in mean age 35-66 years and 16 females in mean age 40-55 mean years) were Streptococcus pneumonia (16 cases), Staphilococcus aureus, (10 cases), Klebsiella pneumonia (5 cases), and other important agents were “atypical”, such as Haemophilus Influenzae, Chlamidophila pneumonie and Moraxela cataralis. A case with Acinetobacter baumani and Proteus Sp. was also widely resistance to mefA gene / ermB gene as all cases of analyzed. The more frequency of genes resistant (29 cases) are ermA gene / ermC / ermB for Staphilococcus aureus and the gene tem+shv / gene / ctx-M with the Chromosomal mutation (7 cases), as gyrA83_87 Ecoli / Pseu for Klebsiella pneumonia agents. Also most resistance antibiotics were Makrolides, (29 cases and Lincosamides (6 cases) and this cases have had the chromosomial integrates. The most resistance microbe, Pseudomonas aeruginosa(1 case), has been registered as multi drugs resistance [MDR]*. Conclusion: The Unyvero™ results have been available 2 days before the primary microbiology report and 3 days before the final confirmation results, obtained by microbiology culture. The Unyvero Analyzer only provides rapid data to support the therapeutic decision of currant medic.
Antibiotic Resistance, PCR, Gene, DNA, Resistance Markers, Microbiology Report
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