Effect of Plasmid Curing on Antibiotic Susceptibility: A Study on Bacterial Isolates from Cases of Otitis media
[1]
Orhue O. P., Department of Microbiology, Faculty of Natural Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
[2]
Ehidiamen G. N., Department of Microbiology, Faculty of Natural Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
[3]
Momoh A. R. M., Department of Medical Microbiology, Faculty of Clinical Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
[4]
Akpamu U., Department of Physiology, Faculty of Basic Medical Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
In this part of the world, Otitis media is still a challenging infection. Its continuous antimicrobial prophylaxis has become problematic due to the threat of antimicrobial resistance; making the spread of drug resistance one of the most serious threats to it successful treatment. The aim of this study is to determine the effect of plasmid curing on antibiotic susceptibility pattern of bacterial isolate from Otitis media to some selected antibiotics. The study involved 16 positive samples at the Ear Nose and Throat (ENT) unit of University of Benin Teaching Hospital between 1st August and 31st October 2011. Using standard procedures, the susceptibility pattern of the bacterial isolates to some selected antibiotics were done pre and post plasmid curing. The results were then compared and the percentage effect of plasmid curing was calculated and compared using simple descriptive statistics of frequencies and percentages. Antibiotics resistance of isolates showed multidrug resistance to Ampiclox, Amoxiclav, Nitrofuratoin, Amoxacillin and Metronidazole pre plasmid curing. Comparatively, there were variations in susceptibility pattern between the pre and post plasmid curing and this occur in different percentages with the different bacterial isolates. The resistance of most of the isolates in this study to antibiotics was plasmid mediated, hence, antibiotics resistance may be due to plasmids. The result of this study may underscore the need for provision of fund for search of new antibiotics that are effective to the emergent and re-emerging strains of resistant bacteria organisms. It is however, recommended that cultures be made from intractable infections of the ear in order to get an appropriate antibiogram of the organism for effective treatment instead of application of blind treatment.
Otitis media, Antibacterial Susceptibility, Antibiotics Resistance, Plasmid Curing
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