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Antibacterial Activity of Khaya senegalensis Stem Bark Extracts Against Some Bacteria Isolated from Poultry Droppings
Current Issue
Volume 2, 2015
Issue 2 (April)
Pages: 31-37   |   Vol. 2, No. 2, April 2015   |   Follow on         
Paper in PDF Downloads: 41   Since Aug. 28, 2015 Views: 1917   Since Aug. 28, 2015
Authors
[1]
Muhammad A. B., Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences Bayero University, Kano, Nigeria.
[2]
Olonitola O. S., Department of Microbiology, Faculty of Sciences, Ahmadu Bello University Zaria, Kaduna, Nigeria.
[3]
Jatau E. D., Department of Microbiology, Faculty of Sciences, Ahmadu Bello University Zaria, Kaduna, Nigeria.
[4]
Yahaya H., Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences Bayero University, Kano, Nigeria.
[5]
Gwarzo M. Y., Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences Bayero University, Kano, Nigeria.
[6]
Aliyu M. S., Department of Microbiology, Faculty of Sciences, Ahmadu Bello University Zaria, Kaduna, Nigeria.
[7]
Doko H. I. M., Department of Microbiology, Faculty of Sciences, Ahmadu Bello University Zaria, Kaduna, Nigeria.
Abstract
Recent findings have incriminated the use of antibiotics in poultry feeds to be associated with emergence of resistance among bacteria. The use of traditional plants remedies has not been found to be associated with resistance. This work determined the inhibitory effects of extract of Khaya senegalensis used in traditional poultry farming against Salmonella species, Escherichia coli, Proteus species, Pseudomonas species and Staphylococcus aureus isolated from poultry dropping. The method of maceration was adopted to extract the plant materials with methanol (70%) and sterile water as solvents for the extraction. One gram each of sample (poultry dropping) was diluted in selenite F broth and another one gram each was inoculated directly into Salmonella – shigella agar, blue green agar, McConkey agar, Manitol salt agar and Eosin methylene blue agar (EMB). After an overnight incubation at 37oC for 24 hrs plates were read. Typical colonies were grams Stained using Standard procedures, consequent upon that, the isolated gram negative organisms were subjected to biochemical test. The only Gram positive organism isolated was further confirmed to be S. aureus by catalase and coagulase tests. Bioassay of the isolates was conducted and further Subjected to the plant extracts, using agar well diffusion method and conventional antibiotics using disc diffusion (Kirby bouer). Findings shows significant effect of the plant extracts on the isolated bacteria and also indicated that it can serve as an alternative to conventional antibiotics as used in traditional poultry production in Nigeria. This practice can also go a long way in minimizing Emergence of resistance strains of bacteria in human diseases since drugs derived from plants are safe and dependable, compared to synthetic drugs.
Keywords
Khaya senegalensis, Antibacterial, MacConkey Agar, Kirby Bouer, Poultry Droppings
Reference
[1]
Abalaka, M.E. (2003). Studies on the antimicrobial activity of bark extracts of Khaya senegalensis. An M.Sc. Thesis, University of Ilorin, Nigeria.
[2]
Abdu, P. A. and Faya, J. N. (2000). The efficacy of some Nigerian plants on helminths found in local chickens. Ethnoveterinary practices research and development. Proceedings of an International Workshop on Ethnoveterinary Practices held 14-18 August 2000, Kaduna, Nigeria. Pp. 65-71.
[3]
Abdu, P. A., Sa’idu, L. and George, B. D. J. (2000). Diseases of local poultry in Nigeria. Discovery and Innovation, 14(1/2): 107-118.
[4]
Ahmed, I., Olonitola, O.S., Yakubu, S.E. and Ado, S.A. (2014).characterization and susceptibility of Escherichia coli isolated from infantile diarrhea to momordica charantia and Aeschynomene uniflora extracts in kano state, Nigeria. A Ph.D thesis, Ahmadu Bello University, zaria, Nigeria.
[5]
Akiyama,H., Kazuyasu, F., Yamsaki, O., Oono, T. and Iwatsuki, K. (2001). Antibacterial action of several Tannins against Staphylococcus aureus. Journal of Antimicrobial chemotherapy, 48(48): 487-491.
[6]
Akortha, E.E., Aluyi, H.S.A. (2002). Plasmid mediated antibiotic resistance among some members of the enterobacteraceae from diarrhoeal patients. Trop. J. Environ. Sci. Health, 5(1): 13 -16.
[7]
Alders, R. and Spradbrow, P. B. (2001).Controlling Newcastle in village chicken. A field Manual Australian Center for International Agriculture.
[8]
Allen, P.C., Danforth, H.D., and Augustine, P.C. (1998). Dietary modulation of avian coccidiosis.Int. J. Parasitolog.28:1131-1140.
[9]
Andrews, M.J. (2004). Determinative of MIC.Department of Microbiology, City Hospital NHS Trust, Birmingham B 187 QH.
[10]
Banso, A and Adeyemo, S.O (2007). Evaluation of antibacterial properties of tannins isolated from Dichrostachys cinerea. African Journal of Biotechnology. 6 (15), 1785-1787.
[11]
Bauer, A.W., Kirby, W. Sherris, J. and Turck, M. (1966).Antibiotic susceptibility testing by a standardized disc method. Animal Journal and Clinical Pathology, 45: 493–496.
[12]
Cavazonni, V., Adami, A. and Castrovilli, C. (1998). Performance of broiler chickens supplemented withBacillus coagulans as probiotic. Broilers and Poultry Science.39:526-529.
[13]
Cheesbrough, M. (2004).District laboratory practice in tropical countries.Part 2.UK; Cambridge University Press.
[14]
Cheesbrough, M. (2006).District laboratory practice in tropical countries.Part 2.UK; Cambridge University Press.
[15]
Clinical Laboratory institute standards (CLIS), (2005). Performance standards for antimicrobial susceptibility 15th informational supplement (CLIS document M100-S15). Vol. 25, Wayne, P.A
[16]
Cos P, Hermans, N., De Bruyne, T., Apers, S., Sindambiwe, J.B., Vanden Berghe, D., Pieters, L. and Vlietinck, A.J.J. (2002). Further evaluation of Rwandan medicinal plant extracts for their antimicrobial and antiviral activities. Ethnopharmacology, 79(2): 155-163.
[17]
Gibson, G.R., and Fulle, R. (2002). Symposium: probiotic bacteria: Implications for human health. Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use. Journal of Nutrition, 130:391S-395
[18]
Gislene, G.F., Locatelli,J., Freitas, P.C.and Silva, G.L. (2000). Antimicrobial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Brazilian Journal of Microbiology. 31 (04): 20-33.
[19]
Ibrahim, T.A, Ajala, L., Adetuyi, F.O. and Ojei, J.B. (2009). Assessment of the Antibacterial activity of Vernonia amygdalina and Occimum gratissimum leaves on selected Food Borne Pathogens. International Journal of Third World Medicine, 8(2): 1539-1545.
[20]
Isyaku, I., Sule, M. and Akujobi, C.O. (2001). Antidiarrhoeal activity of leaf extract of chrozophora senegalensis (Euphorbiacea). Techno science Africana Journal, 2(1): 115-118.
[21]
Kamel, C. (2000). A novel look at a classic approach of plant extracts. Feed Mix 8:16-17.
[22]
Kudi, A.C. and Myint, S.H., (1999). Antiviral activity of some Nigerian medicinal plant extracts. Journal of Ethnophamarcolog. 68: 289-294.
[23]
Kurosoki, F and Nishi, A (1983).Isolation and antimicrobial activity of the phytoalexin–6 methoxymellein from cultured carrot cells. Phytochemistry. 22 (3), 669-672.
[24]
Mapiye, C. and Sibanda, S. (2005). Constraints and opportunities of village chicken production systems in the smallholder sector of Rushinga district of Zimbabwe. Livestock Research for Rural Development.17 (10): 1-7.
[25]
Salisu, L., and Garba, S. (1997). Phytochemical screening and insecticidal activity of Hyptis sauveolens and Striga her monthica. BEST Journal, 5(1): 160-163.
[26]
Sani, A.A.,Emmanuel, T.A., Abdulrahim, R.O., Abdulkareem, S.S., Ramat, A.B. and Ilyas, M. (2012). A study review of documented phytochemistry of Vernonia amygdalina as the basis for pharmacological activity of plant extracts. journal of natural Sciences research. (2)7; 25-57.
[27]
Sherris, J.C., Kenneth, J.R., and george, C.R. (1966). Sherris medical microbiology, an introduction to infectious diseases.4th Edition and revised (2004). McGRAW Hill medical publishing Division, USA.
[28]
Silva, G.L., Lee, I. and Kinghorn, A.D. (2004).In Cannell, R.J.P. Natural products isolation. Methods in bactechnology: Human press inc., Totowa, NJ, 4: 343-363.Sofowora, A. (1993). Medicinal plants and traditional Medicine in Africa. John Wiley and Sons, NewYork, Pp.100-117.
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