A Novel Biosensor for Determination of Glucose Based on MWCNTs/Pt Nanocomposite
[1]
Omar A. Al-Hartomy, Physics Department, Faculty of Science, King Abdul Aziz University, Jeddah 21589, Saudi Arabia.
[2]
M. Mujahid, Department of Physics, Faculty of Science, University of Tabuk, Tabuk, P.O. 741, Tabuk 71491, Saudi Arabia; Senior Secondary School (Boys), Department of Physics, Aligarh Muslim University, Aligarh – 202002, India.
The present study deals with the synthesis of platinum coated multiwalled carbon nanotubes (MWCNTs/Pt). The synthesis was carried out using chemical route. The synthesized particle was characterized using standard analytical techniques such as transmission electron microscopy (TEM) and Raman spectroscopy. The TEM image of MWCNTs, shows a uniform layer of MWCNTs having diameter of about 20–35 nm with the thickness of the coating layer 5–12 nm. After immobilization of platinum nanoparticles (PtNPs), the resulting TEM image reveals that spherical Pt particles are present as dark dots with non-ordered distribution with the mean diameter of 4.5 nm. The Raman spectra shows a peaks of MWCNTs at 1352 cm−1 (D band) and 1584 cm−1 (G band). The observed ratio of the D band to the G band (R value) is from 1.05 to 1.22, associated with the change from MWCNT-COOH to MWCNTs/Pt suggesting that Pt particles have improved the roughness of MWCNTs. The R value of MWCNTs/Pt nanocomposite is reduced to 1.11, which indicates that PtNPs grew in the pores of MWCNTs and some defects disappeared. Since it was reported that MWCNTs/Pt shows good electrochemical activity therefore an attempt has been made to construct glucose biosensor by absorbing glucose oxidase (GOD) on this synthesized material. The result indicates that direct electron transfer process take place at the MWCNTs/Pt/GOD-modified glassy carbon electrode. This biosensor shows good reproducibility, operational stability and has a well storage stability. The results reveal that MWCNTs/Pt/GOD biocomposite could be used in practically oriented application like determination of blood sugar concentration in blood.
Multiwalled Carbon Nanotubes, Biocomposite, Biosensors, Platinum Nanoparticles
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