Spleen Histological Changes Following Monosodium Glutamate Ingestion in Adult Male Wistar Rat
Idehen I. Charles, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
Bankole J. Kayode, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
Airhomwanbor Kingsley, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
DIC-Ijiewere O. Ebenezer, Department of Chemical Pathology, Faculty of Clinical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
Eidangbe A. Peace, Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
This study investigates the effect of ingestion of monosodium glutamate (MSG) on the spleen histology of adult male Wistar rats. The study involved 30 adult rats (140 to 180grams). They were divided into five groups of 6 rats each: A (control; placed on water and rat chow feed), while groups B to E served as the test groups and received chow feed plus solution containing 40mg/kg bwt, 80mg/kg bwt, 120mg/kg bwt and 160mg/kg bwt of MSG respectively daily for 28 days. Their weights were monitored throughout the period of the experiment and standard care for laboratory animal was practiced. At the end of the 28th day, all animals were sacrificed and the spleen harvested following standard laboratory procedures and fixed in 10% formal saline for histological processing. The results showed test groups A and B presented normal spleen histology with no alteration in architectural arrangement of cells. However, test group C and D presented mild fatty degeneration and test group E showed moderate tissue changes. The observations suggest that MSG at high dose may be toxic to the spleen and may induce spleen tissue damages in a dose dependent manner. Based on our findings, doses above 40mg/kg body weight presented adverse effect on the spleen.
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