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Effects of Alcohol and Rohypnol Combination on the Liver Function of Adult Albino Rats
Current Issue
Volume 5, 2018
Issue 3 (June)
Pages: 28-33   |   Vol. 5, No. 3, June 2018   |   Follow on         
Paper in PDF Downloads: 20   Since Jul. 23, 2018 Views: 966   Since Jul. 23, 2018
Authors
[1]
Airhomwanbor Kingsley Osayande, Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Nigeria.
[2]
Dic-Ijiewere Ebenezer Oseremen, Department of Chemical Pathology, Ambrose Alli University, Ekpoma, Nigeria.
[3]
Idehen Iyore Charles, Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Nigeria.
[4]
Osarobo Eseiwi, Department of Haematology, Ambrose Alli University, Ekpoma, Nigeria.
[5]
Igwe R. M. Ndubuisi, Edo State Hospital Management Board, Benin City, Nigeria.
[6]
Osatohanmwen Oghogho, Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Nigeria.
Abstract
This study was aimed at investigating the effect of alcohol and rohypnol on the liver function of Adult albino rats. This study involved 30 albino rats that were divided into five groups, A, B, C, D and E comprising of six (6) albino rats each. Group A served as the control and received only grower mash and water while Group B, C, D and E received 2ml of alcohol and 2ml of 0.5 mg. 1mg, 1.5mg, 2mg and 2.5mg of rohypnol respectively. Significantly lower weight value was obtained. The result obtained showed that alcohol and increasing doses of rohypnol did significantly affected; AST levels of test groups (B=63.0±6.74, C=78.0±7.69, D=109.33±16.65 and E=157.25±4.43) in comparison with control (A=45.0±5.29), mean ALP levels of the test group (B=64.33±9.42, C= 71.0±11.44, D=84.67±11.38 and E=93.0±5.59) when compared with the control group A (50.0±4.32), mean ALT levels of the test group (B=45.33±2.73, C=53.33±3.35, D=55.33±7.02 and E=69.5±5.97) when compared with the control group A (38.5±4.12). There was also a significant increase in the mean total bilirubin in the test group (B=0.48±0.12, C=0.83±0.15, D=1.07±0.15 and E=1.23±0.13) when compared with the control group A (0.38±0.1) and a complementary increase in the mean conjugated bilirubin in the test group (B=0.33±0.1, C=0.48±0.08, D=0.6±0.1 and E=0.9±0.12) when compared with the control group A (0.18±0.05). The results obtained in this study following the administration of alcohol and increasing dosage of rohypnol showed an increase in some liver enzyme markers indicating a significant level of liver damage.
Keywords
Alcohol, Rohypnol, Liver, Wister Rat, Enzyme
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