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Antibacterial Potential of Leave Extract of Different Species of Ocimum (O. Gratisimum & O. Guineas) on Bacterial Isolates from Urinary Tract
Current Issue
Volume 2, 2014
Issue 2 (April)
Pages: 9-13   |   Vol. 2, No. 2, April 2014   |   Follow on         
Paper in PDF Downloads: 27   Since Aug. 28, 2015 Views: 1307   Since Aug. 28, 2015
Authors
[1]
Orhue P. O., Department of Microbiology, Faculty of Natural Sciences; Ambrose Alli University, Ekpoma, Nigeria.
[2]
Momoh A. R. M., Department of Medical Microbiology, Faculty of Clinical Sciences, College of Medicine; Ambrose Alli University, Ekpoma, Nigeria.
[3]
Igumbor E. O., Department of Microbiology, Faculty of Natural Sciences; Ambrose Alli University, Ekpoma, Nigeria.
[4]
Esumeh F. I., Department of Microbiology, Faculty of Natural Sciences; Ambrose Alli University, Ekpoma, Nigeria.
Abstract
Urinary tract infection is a threat to the world population and economic, and the situation is further worsen by the high costs and hazardous side-effects of synthetic drugs in mix of drug resistance. The importance of this study lies in evaluating the antimicrobial potentials of different solvents extracts of Ocimum spp against some human bacteria causing urinary tract infections. Following standard laboratory procedures, the leaves extracts of Ocimum gratisimum and Ocimum guineas were studied on Gram positive bacteria (Staphylococcus aureus) and two strains of Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). The results showed that the petroleum ether and 1%HCl extracts showed high antibiotic potentials that are similar to standard antimicrobial drugs. Worrisome is the observed insensitivity against the urinary tract infection herein studied with the water and ethanolic extracts of Ocimum gratisimum and Ocimum guineas. However, acetone extract in Ocimum guineas was sensitive to S. aureus only. Conclusively, petroleum ether and 1%HCl extracts may served as preferred extraction solvents for Ocimum gratisimum and Ocimum guineas leave considering the inhibitory activities against urinary tract microbes.
Keywords
Herbs, Therapeutic Potential, Antimicrobial, Urinary Tract Infection, Ocimum Species
Reference
[1]
Gonzalez, C.M., Schaeffer, A.J. Treatment of urinary tract infection: what's old, what's new, and what works. World J Urol., 1999; 17(6):372–382.
[2]
Gupta, K. Addressing antibiotic resistance. Dis Mon., 2003; 49(2):99–110.
[3]
Kahlmeter, G. An international survey of the antimicrobial susceptibility of pathogens from uncomplicated urinary tract infections: the ECO.SENS Project. J Antimicrob Chemother. 2003; 51(1):69–76.
[4]
Miller, K., O'Neill, A.J., Chopra, I. Response of Escherichia coli hypermutators to selection pressure with antimicrobial agents from different classes. J Antimicrob Chemother. 2002; 49(6):925–934.
[5]
Essawi, T., Srour, M. J Ethanopharmacol, 2000. 46: 343-349.
[6]
Sofowora, A. Medicinal plants and traditional medicines in Africa. Chichester John, Willey and Sons New York. 1993. p 256.
[7]
Oladunmoye, M.K. Comparative Evaluation of Antimicrobial Activities and Phytochemical Screening of Two Varieties of Acalypha Wilkesiana. Trends Appl. Sci. Res., 2006., 538 – 541.
[8]
Iniaghe, O.M., Malomo S.O., Adebayo J.O., Proximate composition and phytochemical constituents of leaves of some Acalypha species. Pak. J. Nutr., 2009; 8, 256-258.
[9]
Stephen, U.A., Abiodun, F., Osahon, V., Ewaen, V. Phytochemical analysis and antibacterial activity of Khaya grandifoliola stem bark. J. Biol. Sci., 2009; 9, 63-67.
[10]
Akgül, A. Spice Science and Technology. Turkish Association Food Technologists Publ. No. 15, Ankara, Turkey. (in Turkish). 1993.
[11]
Baritaux O., Richard H., Touche J., Derbesy M. Effects of drying and storage of herbs and spices on the essential oil. Part I. Basil, Ocimum basilicum L. Flavour Fragr. J., 1992; 7: 267–271.
[12]
Odugbemi, T. Outlines and pictures of medicinal plants from Nigeria. University of Lagos Press, 2006; 9:102.
[13]
Saliu, B.K., Usman, L.A., Sani, A., Muhammad, N.O., Akolade, J.O. Chemical composition and anti bacterial (oral isolates) activity of leaf essential oil of Ocimum gratissimum L. grown in north central Nigeria. International Journal of Current Research, 2011; 33 (3): 022-028.
[14]
Okwu, D.E. The potentials of Ocimum gratissimum, Pengluria extensa and Tetrapleure atetraptera as spices and flavouring agents. J Chem. Soc. Nigeria, 2006; 31 (1 &2): 38–42.
[15]
Owokotomo, I.A., Ekundayo, O., Dina, O. Ocimum Gratissimum : the Brine Shrimps Lethality of a New Chemotype Grown in South Western Nigeria. Global Journal of Science Frontier Research Chemistry, 2012; 12(6):44-49.
[16]
Effraim, K.D., Jacks, T.W., Sodipo, O.A. Histopathological studies on the toxicity of Ocimum gratissimum leaves extract on some organs of rabbit. African Journal of biomedical Research; 2003; 6: 21-5.
[17]
Akinmoladun AC, Ibukun E.O, Afor E, Obuotor EM. Farombi EO. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sci. Res. Essay, 2007;2:163-166.
[18]
Madeira, S.V.F., Matos, F.J.A., Leal-Cardoso, J.H. Criddle, D.N. Relaxant effects of the essential oil of Ocimum gratissimum on isolated ileum of the guinea-pig. Journal of thnopharmacology, 2002; 81: 1-4.
[19]
Ijeh, I.I., Omodamiro, O.D., Nwanna, I.J. Antimicrobial effects of aqueous and ethanolic fractions of two spices, Ocimum gratissimum and Xylopia aethiopica. African Journal of Biotechnology, 2005; 4: 953-6.
[20]
Lexa, G.M., Josphat, C.M., Francis, N.W., Titus, K.M. Chemical composition and antimicrobial activity of the Essential oil of Ocimum gratissimum L. growing in Eastern Kenya. African Journal of Biotechnology, 2007; 6 (6): 760-765.
[21]
Kokwaro, J.O. Medicinal plants of East Africa, East Africa Literature Bureau, Kampala, Nairobi, and Dar-es-Salaam. 1993; P. 106-115.
[22]
Tania, U., Nakamura, R.R., Mendonca, F. Antileishmanial activity of Eugenol-rich essential oil from Ocimum gratissimum. Parasitol International, 2006; 55: 99-105.
[23]
Orhue .P.O. Antibiogram of some indigenous plant extract susceptibility profile of Europathogenic bacterial isolated from UBTH Benin city. PhD THESIS Ambrose Alli University, Nigeria. 2004
[24]
El-Said, F., Sofowora, E. A., Malcon, S.A., Hofer, A. An investigation into the efficacy of Ocimum gratissimum as used in Nigerian native medicine. Planta Medica., 1969; 18: 173-176.
[25]
Williamson, E.M. Ocimum sanctum. In Major herbs of Ayurveda, London, Churchill Livingstone publication. 2002; 201–205.
[26]
Özcan, M., Chalchat, J.C. Essential oil composition of Ocimum basilicum L. and Ocimum minimum L. in Turkey. Czech J. Food Sci., 2002; 20: 223–228.
[27]
Marjorie, M.C. Plant products as antimicrobial agents. Clin. Microbiol. Rev., 1999; 12(4): 564-582.
[28]
Ngbede, J., Yakubu, R.A., Nyann, D.A. Phytochemical screening for active compounds in canarium schweinfurthii (Atile) leaves from Jos North, Plateau state, Nigeria. Res. J. Biol. Sci., 2008; 3 (9): 1076-1078.
[29]
Kumar, A., Lavarasan, R.I., Jaya chandran, T., Decaraman, M., Aravindhan, P., Padmanabhan, N., Krishnan, M.R.V. Phytochemical investigation on a tropical plant, Syzygium cumini from Kattuppalayam, Erode District, Tamil Nadu, South India. Pakistan J. Nutr., 2009; 8 (1), 83-85.
[30]
Orhue, P.O., Edomwande, E.C., Igbinosa, E., Momoh, A.R.M., Asekomhe, O. Antibacterial activity of extracts of mistletoe (tapinanthus dodoneifollus (dc) dancer) from cocoa tree (theobrama cacao). Inter. J. Herbs Pharmaco. Res., 2014; 3(1): 24 – 29.
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