Ameliorative Effects of Combined Administration of Lycopene and/or Zinc on Biomarkers of Oxidative Stress in Alloxan- Induced Diabetic Wistar Rat
Diabetes mellitus remains a burdensome and public health problem with increasing global. The current global prevalence of diabetes mellitus, 285 million people in 2010, is estimated to be 439. This work was designed to study the effects of combine administration of lycopene and zinc on some biomarkers of oxidative stress in alloxan- induced diabetic rats. Apparently healthy albino rats weighing between 150g and 200g were used. The rats were randomly allotted into six groups, each containing five albino rats respectively. Five of the groups (II, III IV V and VI) were induced with diabetes by single intraperitoneal (i.p) injection of freshly prepared in 0.1 mol/L citrate buffered solution (pH 4.5) of alloxan (Sigma Aldrich, St. Louis, MO, USA) at a dose of 150 mg/kg body weight. Control (vehicle) rats were injected with equal volume of 0.1 mol/L citrate buffer. Four days after alloxan injection, diabetes induction was confirmed by measuring fasting blood glucose level in a tail vein blood samples using ACCU-CHEK compact plus glucometer (Roche, France). Rats with glucose level of 200 mg/dl or higher were considered as diabetic. After the induction of diabetes the rats were treated using the Lycopene and zinc separately and in combination respectively according to group daily, whereas, the other group (I) was not given any treatment and this served as the normal control, providing a baseline data. The results indicated that oral supplementation of lycopene (10 mg/kg b.w /day) and zinc (20mg/kg b.w /day) separately or in combination for 4 weeks of treatment exhibited significant alterations in the alloxan-induced-type-1 diabetes. The result showed that there was an increase in the serum CAT activity, SOD and GPx levels of the group treated with lycopene supplement as compared to the diabetic control group. The result also showed a significant p<0.05 increase in CAT, SOD and GPx levels in the group treated with zinc supplement when compared with the diabetic control group. There was also a significant p<0.05 increase in SOD and GPx levels in the group treated with zinc and lycopene as compared with the diabetic control. There was also a decrease in MDA concentrations for the groups treated with lycopene, zinc and the combined regimen as compared to the diabetic control. In conclusion, our finding indicates better ameliorative effects of combined treatment with lycopene and zinc for the oxidative stress in alloxan-induced diabetic rats.
Diabetes, Alloxan, Lycopene, Zinc, Wistar Rats
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