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Fractionation and Determination of Antioxidant Activities, of the Leaves of Senna Occidentalis Ethanol Extract
Current Issue
Volume 6, 2018
Issue 3 (June)
Pages: 26-30   |   Vol. 6, No. 3, June 2018   |   Follow on         
Paper in PDF Downloads: 25   Since Jul. 5, 2018 Views: 335   Since Jul. 5, 2018
Authors
[1]
Ojochenemi Ejeh Yakubu, Department of Biochemistry, Federal University, Wukari, Nigeria.
[2]
Olawale Otitoju, Department of Biochemistry, Federal University, Wukari, Nigeria.
[3]
Edobor Peter Kenneth Imarenezor, Department of Microbiology, Federal University, Wukari, Nigeria.
[4]
Silas Verwiyeh Tatah, Department of Biochemistry, Federal University, Wukari, Nigeria.
[5]
Bilyaminu Habibu, Department of Biochemistry, Federal University, Wukari, Nigeria.
Abstract
Senna occidentalis has been reported to have certain medicinal properties in traditional settings. Extraction was carried out using absolute ethanol and elution was done with the following solvent; beginning from chloroform, ethyl acetate, methanol and finally water. The in vitro antioxidant activity, total flavonoid and total phenolic contents of nine (9) fractions of Senna occidentalis leaves ethanol extract were determined using spectrophotometric methods, the fractions obtained from the extract were concentrated based on the solvent combination for nine fractions, and the Total antioxidant capacity of the fractions expressed in mg/ml of trolox equivalent (TE) which ranged from 43 – 127 mg/ml, Total flavonoids content were expressed as quercetin equivalent (QE) ranging from 104 – 142 mg/ml, and Total polyphenol content expressed as Gallic acid equivalent (GAE) which ranged from 139 – 206. Water fraction possessed the highest antioxidant activity followed by methanol: water fraction. Linear correlation between TFC/TAC showed strongest positive correlation (R2=0.8092) among all, followed by TPC/TAC (R2=0.6938) and TPC/TFC (R2=0.6284). This relationship indicates that the flavonoids content of the extract is basically responsible for the elicited antioxidant activities (Especially the methanol: water fraction).
Keywords
Fractionation, Antioxidant, Phenolics, Flavonoids, Senna occidentalis
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