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Effects of Salvadora persica L. (Miswak) Aqueous Extract on Some Physiological Indices of Female Albino Rats
Current Issue
Volume 3, 2015
Issue 5 (October)
Pages: 50-55   |   Vol. 3, No. 5, October 2015   |   Follow on         
Paper in PDF Downloads: 41   Since Sep. 8, 2015 Views: 1105   Since Sep. 8, 2015
Authors
[1]
Eman G. E. Helal, Department of Zoology, Faculty of Science, Al-Azhar University (Girls), Nasr City, Cairo, Egypt.
[2]
Hany N. Yousef, Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Roxy, Cairo, Egypt.
Abstract
Objective: Salvadora persica (Miswak) is one of the folk medicinal plants commonly used by some women to delay menses. This study was conducted to evaluate the physiological effects-induced in female albino rats after administration of Salvadora persica aqueous extract. Methods: 20 female rats weighing between 160-180 g were randomized into two groups of 10 animals each. Group 1 (Control group) was orally administered with distilled water (1 ml/100 g bw) while Groups 2 (Miswak-treated group) was orally administered with 7 mg/100 g bw of the plant aqueous extract for 30 consecutive days. Estrus cycle pattern was monitored by daily observation of vaginal smears whereas glucose, total proteins, albumin, globulin, lipid profile, liver function and kidney function indices in addtion to reproductive hormones (estradiol and progesterone) and tumor markers (CA 15-3 and CA-125) were determined in sera at the end of the experiment. Results: In comparison to the control group, S. persica aqueous extract significantly elevated the level of high-density lipoprotein cholesterol (HDL) and decreased serum glucose, total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL) and TC/HDL and LDL/HDL ratios while the values of body weight, liver function indices, kidney function indices and the estimated tumor markers in the extract treated group were approximately similar to those of control animals. Furthermore, Miswak aqueous extract showed adverse effects on sexual hormones, by reducing estradiol and increasing progesterone levels accompained with an irregular estrous cycle with shortened proestrus, estrus and metestrus phases and with lengthened diestrus phase. Conclusions: Overall, Miswak aqueous extract is safe as a natural agent for postponement of menstruation and can be used as a contraceptive plant.
Keywords
Miswak, Salvadora persica, Rats, Physiological Parameters, Tumor Markers
Reference
[1]
Elvin-Lewis M. 1980. Plants used for teeth cleaning throughout the world. J Prev Dent 6: 61-70.
[2]
Eid MA, Al-Shammery AR, Selim HA. 1990. The relationship between chewing stick (miswak) and periodontal health. II Relationship to plaque, gingivitis pocket depth and attachment loss. Quintessence Int 21: 1019-1022.
[3]
Al-Sadhan RI, Almas K. 1999. Miswak (chewing stick): A cultural and scientific heritage. Saudi Dent J 11: 80-88.
[4]
Elvin-Lewis M. 1982. The therapeutic potential of plants used in dental folk medicine. Odontostomatol Trop 5:107-117.
[5]
Kamil M, Jayaraj AF, Ahmed F, Gunasekhar C, Samuel S, et al. 1999. Pharmacognostic and phytochemical studies on Salvadora persica. J Pharm Pharmacol 227: 51-58.
[6]
Hardie J, Ahmed K. 1995. The miswak as an aid in oral hygiene. J Philipp Dent Assoc 47: 33-38.
[7]
Durosinlorun AM, Raji HO, Ayoola N, et al. 2012. Menstrual suppression among female Nigerian pilgrims during Hajj. J Fam Plann Reprod Health Care 38: 270-271.
[8]
Tietz NW. 1995. Clinical Guide to Laboratory Tests, 3rd ed. WB Saunders company, Philadelphia; 518-522.
[9]
Schumann G, Klauke R. 2003. New IFCC refer-ence procedures for the determination of catalytic activity concentrations of five enzymes in serum: preliminary upper reference limits obtained in hospitalized subjects. Clin Chem Acta 327: 69-79.
[10]
Burtis CA, Ashwood ER, Bruns DE. 2006. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 4th ed. Philadelphia: WB Saunders.
[11]
Henry RJ, Cannon DC and Winkelman JW. 1997. Clinical Chemistry Principles and Tetchiness, New York: Harper and Row; p.1440.
[12]
Fossati P, Principe L. 1982. Serum triglycerides determined calorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem 28: 2077-2080.
[13]
Burstein RF, Scholnick VS. 1972. Biochemistry and methodology of lipids. J Lipid Res 25: 375-382.
[14]
Friedewald WT, Levey RI, Fredrickson DS. 1972. Estimation of the concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 18:499-502.
[15]
Marcondes FK, Bianchi, FJ and Tanno, AP. 2002. Determination of the estrus cycle phases of rats: some helpful considerations. Braz J Biol 62 (4A): 609-614.
[16]
Abdoon ASS, Abdel-Rahman HA, Kandil OM, Al-Sagair OA and Mohamed AA. 2014. Effect of Salvadora persica (Miswak) leaves and stem aqueous extracts on ovarian folliculogenesis and uterine histology in female albino rats. Int J phytomed 6: 346-353.
[17]
Amah CI, Yama OE, Noronha CC. 2012. Infecund evaluation of cycling female Sprague-Dawley rats: an aftermath treatment with Momordica charantia seed extract. Middle East Fertil Soc J 17(1):37-41.
[18]
Koneri R, Balaraman R, Saraswati CD. 2006. Antiovulatory and abortifacient potential of the ethanolic extract of roots of Momordica cymbalaria Fenzl in rats. Indian J Pharmacol 38(2):111-114.
[19]
Elaine N. Marieb, Katja Hoehn. 2007. Human anatomy & physiology. 7th ed. San Francisco: Pearson Benjamin Cummings.
[20]
AL-Humesh MJM, Mahmood AY, Lateef RH. 2012. Effect of watery extract for the roots of Salvadora persica on the concentration of the sexual hormones and number of fertility parameters of white mice females. J Tikrit Univ Agric Sci 12 (3): 62-68.
[21]
Smith EL, Hill RL, Leliman IR, Lefkocoitz RJ, Handler P, White A. 1987. Principles of Biochemistry; Mammalian Biochemistry, 7th ed. McGraw-Hill Book Company.
[22]
Ganguly M, Devi N, Mahanta R, Borthakur MK. 2007. Effect of Mimosa pudica root extract on vaginal estrous and serum hormones for screening of antifertility activity in albino mice, Contraception 76 (6): 482-485.
[23]
Oluyemi KA, Okwuonu UC, Baxter DG, Oyesola TO. 2007. Toxic Effects of Methanolic Extract of Aspilia africana Leaf on the Estrous Cycle and Uterine Tissues of Wistar Rats. Int J Morphol 25(3): 609-614.
[24]
Khan M, Ali M, Ali A, Mir SR. 2014. Hypoglycemic and hypolipidemic activities of Arabic and Indian origin Salvadora persica root extract on diabetic rats with histopathology of their pancreas. Int J Health sci 8(1): 45-56.
[25]
Trovato A, Galati EM, Rossitto A, et al. 1998. Hypoglycemic effects of Salvadora persica L. in the rat. Phytomed 5:129-132.
[26]
Fortun-Lamothe L., Drouet-Viard F. 2002. Review: II-Diet and immunity current state of knowledge and research prospects for the rabbit. World Rabbit Sci 10:25-39.
[27]
El-Kholy KH, El-Aasar TA, Abo-Bakr HA. 2008a. Some physiological and immunological responses of rabbits fed diets contained Salvadora persica. Egypt J Rabbit Sci. 18(1): 15-29.
[28]
Pari L, Kumar AN. 2002. Hepatoprotective activity of Moringa Oleifera on antitubercular drug induced liver damage in rats. J Med Food 5:171-177.
[29]
Kim HC, Nam CM, Jee SH, Han KH, Oh DK, Suh II. 2004. Normal serum aminotransferase concentration and risk of mortality from liver diseases: prospective cohort study. BMJ 328: 983-987.
[30]
El-Kholy KH, Seleem TST, El-Aasar TA, Hanaa Abdelharith A. (2008b). Effect of dietary addition of Arak (Salvadora persica) on growth and reproductive performance in Black Baladi rabbit males. World Rabbit Sci., 16: 21-27.
[31]
Ibrahim SAM, Zanaty GA, Abdel-Azim A. 2005. Miswak and Khella as growth promoters in rabbits performance and some physiological aspects. Egypt Poul Sci J 25: 735-748.
[32]
El-Neney BAM, Awaden NB, Ramadan NA, Rizk AM. 2013. The impact of useing different levels of miswak on reproductive performance and immunity system of dokki 4 laying hens. Egypt Poult Sci 33: 865-888.
[33]
Choudhary MI, Naheed S, Jalil S, Alam JM, Atta-ur-Rahman, (2005): Effects of ethanolic extract of Iris Germanica on lipid profile of rats fed on a high-fat diet. J Ethnopharmacol. 98: 217-220.
[34]
Saini S, Yadav JP. 2013. Antidiabetic and antihyperlipidemic effects of ethanolic extract of Salvadora persica L. on alloxan-induced diabetic rats. Pelagia Res Lib 4(3):178-182.
[35]
Galati EM, Monforte MT, Forestieri AM, Miceli N, Bade A, Trovato A. 1999. Salvadora persica L.: hypolipidemic activity on experimental hypercholesterolemia in rat. Phytomed 6(3): 181-185.‏
[36]
Kinosian B, Glick H, Preiss L, Puder KL. 1995. Cholesterol and coronary heart disease: predicting risks in men by changes in levels and ratios. J Inves Med 43: 443-450.
[37]
Wilcox LJ, Borradaile NM, de Dreu LE, Huff MW. 2001. Secretion of hepatocyte apoB is inhibited by the flavonoids, naringenin and hesperetin, via reduced activity and expression of ACAT2 and MTP. J Lipid Res 42(5):725–734.
[38]
Ibrahim AY, El-Gengaihi SE. 2012. Safety Profile of Meswak Root Extract on Liver, Kidney, Sexual Hormones and Hematological Parameters of Rats. Not Sci Biol 4(1): 18-23.
[39]
Keshaviah A, Dellapasqua S, Rotmensz N, Lindtner J, Crivellari D, Collins J, Colleoni M, Thurlimann B, et al. 2006. CA15-3 and alkaline phosphatase as predictors for breast cancer recurrence: A combined analysis of seven International Breast Cancer Study Group trials. Ann Oncol 18 (4): 701-708.
[40]
Osman N, O'Leary N, Mulcahy E, Barrett N, Wallis F, Hickey K, Gupta R. 2008. Correlation of serum CA125 with stage, grade and survival of patients with epithelial ovarian cancer at a single centre. Ir Med J 101(8): 245-247.
[41]
Darmani H, Nusayr T, Al-Hyiasat AS. 2006. Effects of extracts of meswak and Derum on proliferation of Balb/C 3T3 fibroblasts and viability of carcinogenic bacteria. Int J Dent Hyg 4(2): 62-66.
[42]
Ibrahim AY, El-Gengaihi SE, Motawe HM. 2011. Phytochemical and cytotoxicity investigations of Salvadora persica bark extracts. JASMR 6(2): 127-133.
[43]
Mandl AM. 1951. The phases of the oestrous cycle in the adult white rat. J Exp Biol 28: 576-584.
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