Phytochemical Screening and Antioxidant Activity of Justicia tranquebariensis and Bauhinia Racemosa
Introduction: The phytochemicals in medicinal plants are defined as bioactive non nutrient plant compounds in fruits, vegetables and grains that have been linked to reducing the risk of major chronic diseases. The medicinal plant are promising to have such have effective medicinal property due to presence of phytochemical compounds like alkaloids, tannins, flavonoids and phenolic compounds. The present study aimed to find the phytochemicals present in hexane and ethyl acetate extracts of Justicia tranqubarieansis and Bauhinia racemosa and also find out the antioxidant property of these plant extracts. Methods: Phytochemicals present in the hexane and ethyl acetate extract of Justicia tranqubarieansis and Bauhinia racemosa were identified by chemical methods. Antioxidant property was done by DPPH free radical scavenging method. Results: In Justicia tranqubarieansis, hexane extract showed positive for flavonoids, alkaloids and coumarins. Ethyl acetate extract showed positive for flavonoids, alkaloids, cardiac glycosides, terpenoids, coumarins and steroids. Hexane extract of Bauhinia racemosa showed positive for flavonoids, alkaloids, quinines, cardiac glycosides, terpenoids and coumarins. Ethyl acetate extract showed positive for flavonoids, alkaloids, cardiac glycosides, terpenoids, coumarins and steroids. Ethyl acetate showed better extraction of phytochemicals than Hexane. Hexane and ethyl acetate extracts of Justicia tranqubarieansis exhibited 66% and 90% respectively of free radical scavenging activity at a concentration of 150µg. Extracts of Bauhinia racemosa showed less antioxidant activity. Conclusion: This study suggests that the Justicia tranqubarieansis extracts have good antioxidant property, which might be helpful in preventing of slowing they progress of diseases involved as result of oxidative stress.
Alkaloids, Flavonoids, Justicia tranqubarieansis, Antioxidant Activity and Ethyl Acetate
Nagavani V, Rao TR Evaluation of antioxidant potential and qualitative analysis of major polyphenols by RP-HPLC in Nymphaea nouchali Burm flowers. International Journal of Pharmacy and Pharmaceutical Sciences, 2010; 2(Suppl 4): 98-104.
Cartea ME, Francisco M, Lema M, Soengas P, Velasco P Resistance of cabbage (Brassica oleracea capitata Group) crops to Mamestra brassicae. Journal of Economic Entomology, 2010; 103(5): 1866- 1874.
Mundhe KS, Kale AA, Gaikwad SA, Deshpande NR, Kashalkar RV Evaluation of phenol, flavonoid contents and antioxidant activity of Polyalthia longifolia. J Chem Pharm Res,2011;3(1): 764-769.
Hakiman M, Maziah M Non enzymatic and enzymatic antioxidant activities in aqueous extract of different Ficus deltoideaaccessions. Journal of Medicinal Plants Research,2009; 3(3): 120-131.
Chen CC, Hsin WC, Ko FN, Huang YL, Ou JC, Teng CM. J Nat Prod. 1996; 59: 1149.
Bhattarai NK. Fitoterapia 1993; 64: 483.
Panthong A, Kanjanapothi D. J Ethnopharmacol. 1986; 18: 213.
Mahabir D. Am J Pub Health. 1997; 1: 174.
Heinrich M, Kuhnt M, Wright CW, Rimplex H, Phillipson JD, Schandelmaier A, WarHurst DC. J Ethnopharmacol. 1992; 36: 81.
Yangfg LL, Yen KY, Kiso Y, Kikino H. J Ethnopharmacol. 1987; 19: 103.
Nadkarni KM. Indian meteria medica. Vol.1. Bombay Popular Prakashan; p. 715.
Nadkarni KM. Indian materia medica. 3rd ed. Vol. 1. Bombay: Bombay Popular Prakashan; 1982. p. 183.
Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed. Vol. 3. Dehradun: Bishen Singh Mahendra Pal Singh; 1991. p. 894-5.
Anonymous. The Wealth of India. New Delhi: Council of Scientific Research and Industrial Research; 1991. p. 54-5.
Jain R, Alam S, Saxena UA. A new teteacyclic phenol and other constituents from the roots of Bauhinia racemosa. Indian J Chem 2002;41:1321-2.
Prabhakar P, Gandhidasan R, Raman PV, Krishnasamy NR, Nanduri S. A tetracyclic 2,2-dimethyl chroman from the roots of Bauhinia racemosa. Phytochemistry 1994;36:817-8.
Mohan VR, Janardhanan K. Chemical and nutritional evaluation of two germplasms of the tribal pulse, Bauhinia racemosa. Plant Foods Hum Nutr 1994; 96:367-74.
El-Hossary GA, Selim MA, Sayed AE, Khaleel AE. Study of the flavonoids content of Bassia muricata and Bauhinia racemosa. Cairo University: Bulletin of the Faculty of Pharmacy; 2000. p. 56.
Gupta M, Mazumdar UK, Kumar RS, Gomathi P, Rajishwar Y, Kakoti BB, et al. Anti-inflammatory, analgesic and antipyretic effects of methanol extract from Bauhinia racemosa stem bark in animal models. J Ethnopharmacol, 2005;98:267-73.
Akhtar AH, Ahmad KV. Anti-ulcerogenic evaluation of the methanolic extracts of some indigenous medicinal plants of Pakistan in aspirin-ulcerated rats. J Ethnopharmacol 1995;46:1-6.
Aruoma OI. Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutation Res. 2003; 523 (524):9-20.
Dasgupta N, De B. Antioxidant activity of Piper betle L. Leaf extract in vitro. Food Chem. 2004; 88:219-224.
Coruh N, Celep AGS, Ozgokce F. Antioxidant properties of Prangos ferulacea (L) Lindl, Chaerophyllum macropodum Boiss. And Heracleum persicum Desf. From Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food Chem 2007; 100:1237-1242.
Blois MS (1958). Antioxidant determinations by the use of a stable free radical, Nature, 29: 1199-1200.
Sofowora A. Medicinal Plants and Traditional Medicinal in Africa. 2nd Ed. Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd; 1993. Screening Plants for Bioactive Agents; pp. 134–156.
Harborne JB (1973). Phytochemical Methods: A guide to modern techniques of plant analysis. Chapman and Hall, New York, pp. 279, 3rd Edition.
Smolenski SJ, Silinis H, Farnswoth NR (1974). Alkaloids screening. V. Lloydia, 37: 506-536.
Kapoor LD, Singh A, Kapoor SL and Shrivastava SN (1969).Survey of Indian medicinal plants for saponins, alkaloids and flavonoids. Lloydia, 32: 297-302.
Sonali Jana and G.S. Shekhawat, Phytochemical analysis and antibacterial screening of in vivo and in vitro extracts of Indian medicinal herbs: Anethumgraveolens, Research Journal of medicinal plants 4(4): 206-212,2010: ISSN 1819-3455.
GA Ayoola1, HAB Coker1, SA Adesegun2, AA Adepoju-Bello1, K Obaweya1, EC Ezennia1, TO Atangbayila1, Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria, Tropical Journal of Pharmaceutical Research, September 2008; 7 (3): 1019-1024.
C.A.Sureshkumar, R.Varadharajan, P.Muthumani, R. Meera, P.Devi, B.Kameswari.Pharmacognostic and Preliminary Phytochemical Investigations on the stem of Saccharumspontaneum, J. Pharm. Sci. & Res. Vol.1(3), 2009, 129-136.
Manasboxi, Y.Rajesh, V.Rajakumar*, B.Praveen and K.Mangamma. Extraction, phytochemical screening and in-vitro evaluation of anti-oxidant properties of commicarpuschinesis (aqueous leaf extract), International Journal of Pharma and Bio Sciences, Vol.1/Issue-4/Oct-Dec.2010, ISSN 0975-6299.
Kolawole OM, Oguntoye SO, Agbede O and Olayemi AB (2006).Studies on the efficacy of BrideliaferrugineaBenth.bark extract in reducing the coliform load and BOD of domestic waste water. Ethnobotanical Leaflets, 10: 228-238.
Akilandeswari,s., Manimaran, S., Valarmathi, R, Kumar, S.K. and Loganathan, S.V.(2001). Phytochemical observation on leaf of Justicia tranquebariensis L.F.Aceiont science of life 20(3),60-61.
Manohar,P., Rajesham, V.V., M.,Kiran kumar, S. and Prasanna kumara.(2011). Pharmacognostical,phytochemical and antimicrobial activity of Bauhinia racemosa leaves. International Journal of Pharmaceutical Biology 1(1); 10-14.