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Sequence Characteristics of Cinnamate 4-Hydroxylase in Medicinal Plant Astragalus Membranaceus
Current Issue
Volume 5, 2018
Issue 1 (March)
Pages: 15-18   |   Vol. 5, No. 1, March 2018   |   Follow on         
Paper in PDF Downloads: 31   Since Apr. 27, 2018 Views: 1118   Since Apr. 27, 2018
Authors
[1]
Li Gun, Department of Biomedical Engineering, School of Electronic Information Engineering, Xi'an Technological University, Xi'an, China.
Abstract
Astragalus Membranaceus is a kind of commonly used herb in traditional Chinese medicine. The main sequence characteristics of Cinnamate 4-hydroxylase is studied via the bioinformatics tools such as protparam, the protscale system, the TMHMM system and the online Signal peptide analysis tool, et al. The results show that the molecular weight of Cinnamate 4-hydroxylase in Astragalus Membranaceus is 58104.41, its theoretical isoelectric point is 9.20, the instability index is 47.19, phosphorylation sites prediction result show that there are 6 serine sites value close to 1. Finally, this paper summarizes the status and functional usage of Astragalus Membranaceus in the development of traditional Chinese medicine.
Keywords
Cinnamate 4-hydroxylase, Astragalus Membranaceus, Bioinformatics Analysis
Reference
[1]
Xin-Yan Liu, Hai-Na Yu, Shuai Gao (2017). The isolation and functional characterization of three liverwort genes encoding cinnamate 4-hydroxylase. Plant Physiology and Biochemistry, 117: 42-50
[2]
Takahiko Sato, Keiji Takabe, Minoru Fujita (2004). Immunolocalization of phenylalanine ammonia-lyase and cinnamate 4-hydroxylase in differentiating xylem of poplar. Comptes Rendus Biologies, 327 (9-10): 827-836
[3]
Hao Chen, Hanxiao Jiang, John A. Morgan (2007). Non-natural cinnamic acid derivatives as substrates of cinnamate 4-hydroxylase. Phytochemistry, 68 (3): 306-311
[4]
Zhi-yong NI, Bo LI, M Peter Neumann (2014). Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton (Gossypium hirsutum). Journal of Integrative Agriculture, 13 (10): 2102-2112
[5]
Hui Xu, Nam Il Park, Xiaohua Li (2010). Molecular cloning and characterization of phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and genes involved in flavone biosynthesis in Scutellaria baicalensis. Bioresource Technology, 101 (24): 9715-9722
[6]
José María Bellés, María Pilar López-Gresa, Joaquín Fayos (2008). Induction of cinnamate 4-hydroxylase and phenylpropanoids in virus-infected cucumber and melon plants. Plant Science, 174 (5): 524-533
[7]
Huayan Zhao, Jing Lu, Shiyou Lü (2005). Isolation and functional characterization of a cinnamate 4-hydroxylase promoter from Populus tomentos. Plant Science, 168 (5): 1157-1162
[8]
David J. Millar, Marianne Long, Georgina Donovan (2007). Introduction of sense constructs of cinnamate 4-hydroxylase (CYP73A24) in transgenic tomato plants shows opposite effects on flux into stem lignin and fruit flavonoids. Phytochemistry, 68 (11): 1497-1509
[9]
Silvia Hübner, Marc Hehmann, Stephan Schreiner (2003). Functional expression of cinnamate 4-hydroxylase from Ammi majus L. Phytochemistry, 64 (2): 445-452
[10]
Kristopher Blee, Joon W. Choi, Ann P. O’Connell (2001). Antisense and sense expression of cDNA coding for CYP73A15, a class II cinnamate 4-hydroxylase, leads to a delayed and reduced production of lignin in tobacco. Phytochemistry, 57 (7): 1159-1166
[11]
John M. Humphreys, Clint Chapple (2004). Immunodetection and quantification of cytochromes P450 using epitope tagging: immunological, spectroscopic, and kinetic analysis of cinnamate 4-hydroxylase. Journal of Immunological Methods, 292 (1-2): 97-107
[12]
Luo Xiaojiao, Liu Xinchun, Yang Xiaoyun (2014). Cloning and Tissue Expression Analysis of HvC4H Gene in Hulless Barley. Journal of Plant Genetic Resources, 15 (3): 589-596
[13]
Hu Yongsheng, Zhang Lei, Chen Wansheng (2015). Molecular cloning and expression analysis of cinnamic acid 4-hydroxylase gene from Isatis indigotica. Chinese Traditional and Herbal Drug, 46 (1): 101-105
[14]
Fan Bingyou, Li Fang (2013). Cloning and Analysis of Gene Fragments Encoding C4H and CAD from Miscanthus sinensis. Genomics and Applied Biology, 32 (3): 319-324
[15]
Gasteiger E, Hoogland C, Gattiker A (2005). Protein Identification and Analysis Tools on the ExPASy Server, Proteomic Protocols Handbook, 112: 571-607
[16]
Xie Yong, Hong Xiaokun, Yan Renxiang (2017). Bioinformatics analysis of the recombinant rAgaN3 gene of agarase, Chinese Journal of Bioinformatics, 15 (1): 16-26
[17]
Petersen T N, Brunak S, Heijne G (2011). SignalP 4.0: discriminating signal peptides from transmembrane regions. Nature Methods, 8 (10): 785-786
[18]
David T. Jones, Daniel W. A. Buchan, Domenico Cozzetto (2012). PSICOV: precise structural contact prediction using sparse inverse covariance estimation on large multiple sequence alignments. Bioinformatics, 28 (2): 184-90
[19]
Wafa Tombari, Abdeljelil Ghram (2016). Production of a truncated recombinant HA1 for influenza A H9 subtype screening. Biologicals, 44 (6): 546-555
[20]
Antoine Gravot, Romain Larbat, Alain Hehn (2004). Cinnamic acid 4-hydroxylase mechanism-based inactivation by psoralen derivatives: cloning and characterization of a C4H from a psoralen producing plant—Ruta graveolens—exhibiting low sensitivity to psoralen inactivation. Archives of Biochemistry and Biophysics, 422 (1): 71-80
[21]
Zhang Lin, Lin Yiqun, Fu Yanling (2015). Analysis on Clinical Dosage of Huangqi [Astragalus membranaceus] in the Past Dynasties. Journal of Traditional Chinese Medicine, 56 (6): 518-521
[22]
Zhang Qiang, Gao Wenyuan, Man Shuli (2012). Chemical composition and pharmacological activities of Astragali Radix. China Journal of Chinese Materia Medica, 37 (21): 3203-3207
[23]
Zhou Chao, He Yi, Lu Jing (2014). Studies on the preparation of isoflavones reference extract and application on the assay of Astragalus Radix. Chinese Journal of Pharmaceutical Analysis, 34 (3): 523-527
[24]
Liu Xiaohua, Lin Xingyao, Liang Jin (2013). Spectrum-effect Relationship in Diuretic Effect of Astragali Radix. Chinese Journal of Modern Applied Pharmacy, 30 (5): 491-495
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