Anthelminthic Activity Against Sheep Gastrointestinal Nematodes in Chemical Compounds from Tephrosia Vogelii Leaves
The objectives of this work, were to evaluate the in vitro anthelminthic activity of fractions isolated from total ethanol extract of Tephrosia vogelii leaves on the inhibition of egg hatching from sheep´s gastrointestinal nematodes and isolate, determine and identify the chemical compounds in the most active fractions. The in vitro anthelminthic activity of the fractions isolated from ethanol extract of leaves was evaluated in hatching eggs of gastrointestinal nematodes isolated from one sheep naturally infecting. Fractionation, isolation and structural elucidation of the compounds from the most active fractions were carried out by conventional chromatography, mass spectrometry and proton nuclear magnetic resonance (H1 NMR) spectroscopy. The seven fractions isolated from the ethanol extract of T. vogelii leaves, using chromatography showed anthelminthic activity in the egg-hatching inhibition test with regards to the concentration. Triterpenoids, rotenoids and other structures were isolated or determined in the most active fractions. Tephrosin, 12a-Hydroxy-α-toxicarol and deguelin were the major rotenoid compounds. From these results it can be concluded that the ethanol extract from T. vogelii with a promising active extract can be an alternative to be used as chemical anthelminthic medicament in the treatment of gastrointestinal nematodes in sheep.