Welcome to Open Science
Contact Us
Home Books Journals Submission Open Science Join Us News Unsubscribe Page
Anthelminthic Activity Against Sheep Gastrointestinal Nematodes in Chemical Compounds from Tephrosia Vogelii Leaves
Current Issue
Volume 6, 2019
Issue 1 (January)
Pages: 8-17   |   Vol. 6, No. 1, January 2019   |   Follow on         
Paper in PDF Downloads: 10   Since Apr. 9, 2019 Views: 88   Since Apr. 9, 2019
Authors
[1]
Armindo Paixão Antonio, Faculty of Veterinary Medicine, Faculty of Agrarian Sciences, Huambo-University José Eduardo dos Santos, Huambo, Angola.
[2]
Luz María Sánchez Perera, Department of Biopharmaceutical Development, Centre for Animal and Plant Health, CENSA, Mayabeque Cuba.
[3]
Javier Arece García, Estación Experimental de Pastos y Forrajes Indio Hatuey, Matanzas, Cuba.
[4]
Mujeeb Ur Rehmana, H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Science, University of Karachi, Pakistan.
[5]
Muhammad Iqbal Choudhary, H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Science, University of Karachi, Pakistan.
Abstract
The objectives of this work, were to evaluate the in vitro anthelminthic activity of fractions isolated from total ethanol extract of Tephrosia vogelii leaves on the inhibition of egg hatching from sheep´s gastrointestinal nematodes and isolate, determine and identify the chemical compounds in the most active fractions. The in vitro anthelminthic activity of the fractions isolated from ethanol extract of leaves was evaluated in hatching eggs of gastrointestinal nematodes isolated from one sheep naturally infecting. Fractionation, isolation and structural elucidation of the compounds from the most active fractions were carried out by conventional chromatography, mass spectrometry and proton nuclear magnetic resonance (H1 NMR) spectroscopy. The seven fractions isolated from the ethanol extract of T. vogelii leaves, using chromatography showed anthelminthic activity in the egg-hatching inhibition test with regards to the concentration. Triterpenoids, rotenoids and other structures were isolated or determined in the most active fractions. Tephrosin, 12a-Hydroxy-α-toxicarol and deguelin were the major rotenoid compounds. From these results it can be concluded that the ethanol extract from T. vogelii with a promising active extract can be an alternative to be used as chemical anthelminthic medicament in the treatment of gastrointestinal nematodes in sheep.
Keywords
Tephrosia vogelii, in vitro Anthelminthic Activity, Rotenoids, Triterpenes, Chemical Compound Elucidation
Reference
[1]
The objectives of this work, were to evaluate the in vitro anthelminthic activity of fractions isolated from total ethanol extract of Tephrosia vogelii leaves on the inhibition of egg hatching from sheep´s gastrointestinal nematodes and isolate, determine and identify the chemical compounds in the most active fractions. The in vitro anthelminthic activity of the fractions isolated from ethanol extract of leaves was evaluated in hatching eggs of gastrointestinal nematodes isolated from one sheep naturally infecting. Fractionation, isolation and structural elucidation of the compounds from the most active fractions were carried out by conventional chromatography, mass spectrometry and proton nuclear magnetic resonance (H1 NMR) spectroscopy. The seven fractions isolated from the ethanol extract of T. vogelii leaves, using chromatography showed anthelminthic activity in the egg-hatching inhibition test with regards to the concentration. Triterpenoids, rotenoids and other structures were isolated or determined in the most active fractions. Tephrosin, 12a-Hydroxy-α-toxicarol and deguelin were the major rotenoid compounds. From these results it can be concluded that the ethanol extract from T. vogelii with a promising active extract can be an alternative to be used as chemical anthelminthic medicament in the treatment of gastrointestinal nematodes in sheep.
Open Science Scholarly Journals
Open Science is a peer-reviewed platform, the journals of which cover a wide range of academic disciplines and serve the world's research and scholarly communities. Upon acceptance, Open Science Journals will be immediately and permanently free for everyone to read and download.
CONTACT US
Office Address:
228 Park Ave., S#45956, New York, NY 10003
Phone: +(001)(347)535 0661
E-mail:
LET'S GET IN TOUCH
Name
E-mail
Subject
Message
SEND MASSAGE
Copyright © 2013-, Open Science Publishers - All Rights Reserved