Influence Allogeneic Mesenchymal Stem Cells on the Tumour Growth Parameters and Metastatic Potential in the Transplantable Carcinoma Lung Lewis
It was investigated the effect of allogeneic mesenchymal stem cell biological characteristics of the primary tumor, metastasis in male mice C57BL/6, which transplanted cell line Lewis lung carcinoma. It was studied the effect of allogeneic MSCs on tumor weight and volume metastasis in tumor-bearing animals, on level of apoptotic cells and tumor cells distribution in cell cycle phases. The primary culture was obtained from transplantable Lewis lung carcinoma. Apoptotic level was measured by flow cytometry, tumor and metastasis volume were assessed by routine methods of experimental oncology, and level of tumor infiltration by lymphocytes was estimated after their fractionation in ficoll-triombrast gradient. We have shown that intravenous administration of allogeneic mesenchymal stem cells after 8 day transplantation of carcinoma lung Lewis leads to enhance of tumor growth in 1.5 times, tumor infiltration by lymphocytes, volume of metastasis in 3 times, and influences on biological characteristics of primary tumor cells, and it leads to an increase in aneuploidy index (in 1,3 times) and mitotic index and reduced levels of apoptotic cells.
Allogeneic MSCs, Cell Cycle Phases, Apoptosis, Tumor, Metastasis
Ramasamy R., Lam E.W., Soeiro I., Tisato V., Bonnet D., Dazzi F. Mesenchymal stem cells inhibit proliferation and apoptosis of tumor cells: impact on in vivo tumor growth. Leukemia 2007;21(2):304-10.
Cherkashyna D.V., Lebedinskii A.S., Buchanan J.A., Shtemenko N.I, Petrenko A.U. Guerin carcinoma growth inhibition in rats after administration of mesenchymal stromal cells of human adipose tissue and bio-regulators of stem and progenitor cells. J. A. Med. Sc. of Ukraine 2010;16(3):492-506.
T. Zhang, Y. W. Lee, Y. F. Rui, T. Y. Cheng, X. Hua Jiang and G. Li Bone marrow-derived mesenchymal stem cells promote growth and angiogenesis of breast and prostate tumors Stem Cell Research & Therapy 2013, 4:70, doi:10.1186/scrt221.
Mandel K, Yang Y, Schambach A, Glage S, Otte A, Hass R Mesenchymal stem cells directly interact with breast cancer cells and promote tumor cell growth in vitro and in vivo. Stem Cells Dev. 2013 Dec 1;22(23):3114-27. doi: 10.1089/scd.2013.0249. Epub 2013 Sep 9
K. Suzuki, R. Sun, M. Origuchi, M. Kanehira, T. Takahata, J. Itoh, A. Umezawa, H. Kijima, S. Fukuda, Y. Saijo Mesenchymal Stromal Cells Promote Tumor Growth through the Enhancement of Neovascularization/ Mol Med. 2011 Jul-Aug; 17(7-8): 579–587. doi: 10.2119/molmed.2010.00157
Sun Z, Wang S, Zhao RC The roles of mesenchymal stem cells in tumor inflammatory microenvironment//J.Hematol Oncol 2014 Feb 6;7(1):14. doi: 10.1186/1756-8722-7-14.
Huang WH, Chang MC, Tsai KS, Hung MC, Chen HL, Hung SC. Mesenchymal stem cells promote growth and angiogenesis of tumors in mice. Oncogene. 2013 Sep 12;32(37):4343-54. doi: 10.1038/onc.2012.458. Epub 2012 Oct 22.
Guide for the Care and Use of Laboratory Animals. – Washington, D.C., National Academy of Press, 1996. – 140 p
Pyaskovskaya O.N., DasyukevichO.I., GarmanchukL.V., Kolesnik D.L., TodorI.N., Solyanik G.I. Changes in VEGF level and tumor growth characteristics during Lewis lung carcinoma progression towards cis-DDP resistance// Experimental oncology 2007; 29(3):197-202
T. Mosmann, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays, J Immunol Methods 65 (1983) 55–63.
Nicoletti I., Migliorati G., Pagliacci M.C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry // J. Immunol. Methods 1991;139( 2):271-280
Mazurkevich A.Y., Kladnytska L.V., Kovpak V.V. Features conditions selection and cultivation mouse bone marrov adhesive fraction mononuclear cells// Bulletin of Taras Shevchenko National University of Kyiv / Vestnik; 2013, Vol. 64 Issue 2, p41-43
Wels J, Kaplan RN, Rafii S, Lyden D. Migratory neighbors and distant invaders: tumor-associated niche cells. Genes Dev. 2008;22:559–74
Uma N. M. Rao, MD, Sandra J. Lee, ScD, Weixiu Luo, MS, Martin C. Mihm Jr, MD, John M. Kirkwood, MD Presence of Tumor-Infiltrating Lymphocytes and a Dominant Nodule Within Primary Melanoma Are Prognostic Factors for Relapse-Free Survival of Patients With Thick (T4) Primary Melanoma /Am J Clin Pathol 2010;133:646-653, DOI: 10.1309/AJCPTXMEFOVYWDA6
Zhang F, Zhao D, Wang S, Hong L, Li Q. Aneuploidy directly contribute to carcinogenesis by disrupting the asymmetric division of adult stem cells". Medical Hypotheses 2007 68 (1): 237–8. doi:10.1016/j.mehy.2006.06.007