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Effects of Long-Term Fluorogestone Acetate Treatment Combined with PMSG on Oestrus Synchronization and Fertility in Guirra Ewes
Current Issue
Volume 1, 2014
Issue 4 (November)
Pages: 25-29   |   Vol. 1, No. 4, November 2014   |   Follow on         
Paper in PDF Downloads: 22   Since Aug. 28, 2015 Views: 954   Since Aug. 28, 2015
Authors
[1]
Francisco Marco-Jiménez, Laboratory of Biotechnology of Reproduction, Polytechnic University of Valencia, 46022, Valencia, Spain.
[2]
José Salvador Vicente, Laboratory of Biotechnology of Reproduction, Polytechnic University of Valencia, 46022, Valencia, Spain.
[3]
María Pilar Viudes-de-Castro, Animal Technology Center, Valencian Institute of Agrarian Research, 12400, Segorbe, Spain.
Abstract
The first objective was to asses the fertility of Guirra sheep using a standard artificial insemination procedure. In a second step, arising from the first objective, were studies the residual levels of plasma progesterone after treatment with intra-vaginal sponges impregnated with fluorogestone acetate (FGA) and determine the effect upon estrus incidence, interval to estrus onset and pregnancy rate. Estrous synchronization was carried out with FGA sponges (30 mg) for 12 days plus 400 IU eCG administered i.m. at sponge withdrawal. Ewes were AI at 54±1 h using fresh semen deposited in the entrance of the cervix. Blood samples were collected at day 19 ewes after AI, for determination progesterone concentration (P4). Ewes with a concentration of more than 1 ng/ml P4 were considered to be pregnant. In a second experiment, 70 ewes were randomly divided into 2 groups (T1 and T2) and synchronized as previous, but ewes in the second group were synchronized 6 days later than T1. Twelve days after sponge withdrawal for T2, all ewes were divided into 7 lots. One ram was introduced to each lot. Ewes for the T1 were mated at next natural estrous cycle while T2 group ewes were mated at the synchronized estrous and the next natural estrous. During all period, the ewes and rams sexual behavior was recorded by video camera. The ewes were considered in estrous when they were directly observed to accept a ride from a ram (standing reflex). The fertility examined the AI was the 24.7%. Similar percentage of ewes exhibiting estrous were observed (for natural or synchronized) but onset of estrous during the natural estrous was much shorter. The conception rate was higher for T1 compared with T2 (P<0.05) and significant differences were obtained when type of estrous were compared (P<0.05). It was concluded that a detrimental effect of f FGA sponge used for estrous synchronization on fertility in Guirra breed sheep was observed.
Keywords
Ewes, Estrous Synchronization, Fertility, Progesterone, FGA
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