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In Vitro Screening for Salt Tolerance in Aromatic Rice Genotypes
Current Issue
Volume 1, 2014
Issue 2 (September)
Pages: 28-32   |   Vol. 1, No. 2, September 2014   |   Follow on         
Paper in PDF Downloads: 14   Since Aug. 28, 2015 Views: 1322   Since Aug. 28, 2015
Authors
[1]
Md. Nur Zahid, Giant Agro Processing Limited, Bangladesh.
[2]
Mehfuz Hasan, Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh.
[3]
Md. Adil, Bangladesh Rice Research Institute, Bangladesh.
[4]
M. Mofazzal Hossain, Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh.
[5]
M. A. Khaleque Mian, Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh.
Abstract
Dehusked seeds of ten aromatic rice varieties along with a control variety Pokkali were placed on MS media supplemented with NaCl, Na2SO4 and KCl salts at four different levels (0%, 0.2%, 0.4% and 0.6%) to observe the callus induction and plant regeneration responses. For callus induction MS medium was supplemented with 2 mg/L 2,4 D while for plant regeneration MS medium was supplemented with 1 mg/L NAA and BAP. All the genotypes showed significant variation in callus induction and plant regeneration responses. Callus induction and plant regeneration percentage decreased with the increase of salt doses. Shakkhorkhora appeared as the best variety in terms of plant regeneration in most of the cases at different levels of the salts (0-0.6%) while Basmati showed its inferiority for callus induction and plant regeneration. It was concluded that salt tolerant aromatic rice varieties can be obtained employing salt stress in in vitro condition.
Keywords
Aromatic Rice, Salt Stress, Somaclonal Variation
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