In Vitro Screening for Salt Tolerance in Aromatic Rice Genotypes
Dehusked seeds of ten aromatic rice varieties along with a control variety Pokkali were placed on MS media supplemented with NaCl, Na2SO4 and KCl salts at four different levels (0%, 0.2%, 0.4% and 0.6%) to observe the callus induction and plant regeneration responses. For callus induction MS medium was supplemented with 2 mg/L 2,4 D while for plant regeneration MS medium was supplemented with 1 mg/L NAA and BAP. All the genotypes showed significant variation in callus induction and plant regeneration responses. Callus induction and plant regeneration percentage decreased with the increase of salt doses. Shakkhorkhora appeared as the best variety in terms of plant regeneration in most of the cases at different levels of the salts (0-0.6%) while Basmati showed its inferiority for callus induction and plant regeneration. It was concluded that salt tolerant aromatic rice varieties can be obtained employing salt stress in in vitro condition.
Aromatic Rice, Salt Stress, Somaclonal Variation
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