Welcome to Open Science
Contact Us
Home Books Journals Submission Open Science Join Us News
Genetic Polymorphism in Bangladeshi Aromatic Rice through RAPD Analysis
Current Issue
Volume 1, 2014
Issue 2 (September)
Pages: 23-27   |   Vol. 1, No. 2, September 2014   |   Follow on         
Paper in PDF Downloads: 11   Since Aug. 28, 2015 Views: 1574   Since Aug. 28, 2015
Authors
[1]
Mehfuz Hasan, Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur 1706, Bangladesh.
[2]
Mohammad Sharif Raihan, Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur 1706, Bangladesh.
Abstract
Genetic polymorphism and relationships among 25 aromatic rice varieties of Bangladesh were established using random amplified polymorphic DNA (RAPD) primers. Out of sixty 10-mer RAPD primers screened initially, three were chosen and used in a comparative analysis of different varieties of indigenous Bangladeshi aromatic rice. Of the 24 total RAPD fragments amplified, 4 (16.67%) were found to be shared by individuals of all eight varieties. The remaining 20 fragments were found to be polymorphic (83.33%). Pair-wise estimates of similarity ranged from 0.111 to 0.844. Highest genetic diversity was determined between PL-8 and PL-12 genotypes (0.844). The amount of genetic diversity within aromatic rice germplasm was quite high as determined by the genetic similarity coefficients between genotypes. Genetic similarities obtained from RAPD data were also used to create a cluster diagram. Cluster analysis using an un-weighted pair-group method with arithmetic averages (UPGMA) was used to group the varieties and the 25 aromatic rice genotypes were grouped into 4 clusters where cluster I includes the maximum number of varieties (11). Cluster VI includes minimum number of varieties (3). This Study offered a rapid and reliable method for the estimation of variability between different varieties which could be utilized by the breeders for further improvement of the local aromatic rice varieties.
Keywords
Aromatic Rice (Oryza sativa L.), Genetic Variation, Random Amplified Polymorphic DNA (RAPD), Un-Weighted Pair-Group Method with Arithmetic Averages (UPGMA)
Reference
[1]
Alessandra C, Stefania DG, Giovanni DB, Antonino C, Marilena C. 2009. Molecular characterization of Italian rice cultivars. Eur. Food Res. Technol., 6: 875-882.
[2]
Das BK, Jena RC, Samal KC. 2009. Optimization of DNA isolation and PCR protocol for RAPD analysis of banana / plantain (Musa spp.). Int. J. Agri. Sc., 1: 21-25.
[3]
Karunagoda RP, Bandara CS. 2005. Molecular characterization of some consumer-preferred rice varieties using randomly amplified polymorphic dnas (RAPDs). Sri Lankan J. Agric. Sci., 42: 71 – 80.
[4]
Mani P, Bastin J, Arun kumar R, Ahmed ABA. 2010. RAPD-analysis of genetic variation of four important rice varieties using two OPR primers. ARPN J. Agric. Biol. Sc., 5: 25-31.
[5]
Rabbani MA, Pervaiz ZH, Masood MS. 2008. Genetic diversity analysis of traditional and improved cultivars of Pakistani rice (Oryza sativa L.) using RAPD markers. Elec. J. Biotech. 11(3): 35-45.
[6]
Raghunathachari P, Khanna VK, Singh US, Singh NK. 2000. RAPD analysis of genetic variability in Indian scented rice germplasm (Oryza sativa L.). Curr. Sci., 79: 994-998.
[7]
Rasheed S, Fatima T, Husnain T, Bashir K, Riazuddin S. 2005. RAPD characterization of somaclonal variation in indica basmati rice. Pak. J. Bot., 37: 249-262.
[8]
Saker MM, Youssef SS, Abdallah NA, Bashandy HS, Sharkawy AME. 2005. Genetic analysis of some Egyptian rice genotypes using RAPD, SSR and AFLP. African J. Biotech., 4: 882-890.
[9]
Shivapriya M, Shailaja H. 2006. Detection of genotype specific fingerprints and molecular diversity of selected Indian locals and landraces of rice (Oryza sativa L.) using DNA markers. Indian J. Genet. Plant Breed., 66(1): 1-5.
[10]
Skaria R, Sen S, Muneer PMA. 2011. Analysis of Genetic Variability in Rice Varieties (Oryza sativa L.) of Kerala using RAPD Markers. Gen. engg. Biotech. J. 24: 30-37.
[11]
Tehrim S, Pervaiz ZH, Rabbani MA. 2012. Molecular characterization of traditional and improved rice cultivars based on random amplified polymorphic DNAs (RAPDs) markers. African J. Biotech., 11: 10297-10304.
[12]
Thomas G, Mohapatra T, Rao AR, Sharma, RP. 2006. Distinguishing Indian commercial wheat varieties using RAPD based DNA fingerprints. Indian J. Biotechnol. 5: 200-206.
[13]
O’Neill R, Snowdon RJ, Kohler W. 2003. Population genetics aspects of biodiversity. Prog. Bot. 64: 115-137.
Open Science Scholarly Journals
Open Science is a peer-reviewed platform, the journals of which cover a wide range of academic disciplines and serve the world's research and scholarly communities. Upon acceptance, Open Science Journals will be immediately and permanently free for everyone to read and download.
CONTACT US
Office Address:
228 Park Ave., S#45956, New York, NY 10003
Phone: +(001)(347)535 0661
E-mail:
LET'S GET IN TOUCH
Name
E-mail
Subject
Message
SEND MASSAGE
Copyright © 2013-, Open Science Publishers - All Rights Reserved