Anatomy Study of Shoot Tips, Shoots, Flowers and Immature Fruits of Salvadora persica in Vitro
[1]
Abobkar A. M. Saad, Botany Department, Sebha University, Faculty of Science, Sebha, Libya.
[2]
Mansur Megna, Botany Department, Sebha University, Faculty of Science, Sebha, Libya.
Shoot tips, Shoots, flowers and immature fruits were used as explants. They were inoculated on MS medium supplemented with different concentrations of some growth hormones. In case of using shoot tips on MS +NAA (0.1 mg/l) + BA (1mg/l), groups of parenchyma cells were formed meristematic centers and small buds were regenerated after 8 weeks of culture. When they cultured on MS+NAA (0.1 mg/l) + BA (1.5mg/l) small parenchyma cells contain prominent nuclei, tracheids were observed. Shoots on MS+Z (0.5mg/L) + AC (0.5mg/l) only small size of parenchyma cells surrounded by thin cell wall. When flowers were cultured on MS+ NAA (0.5mg/l) + BA (0.5mg/l) different sizes and shapes of parenchyma cells and tracheids were detected. For immature fruits on MS+2,4-D (0.5mg/l) small size of parenchyma cells contain thin cell wall and schlerenchyma cells were seen, On MS+NAA (0.5) + BA ( 1mg/l) only small parenchyma cells surrounded by thin cell wall were observed.
Savadora perscia, Shoot Tips, Shoots, Flower, Immature Fruits, In Vitro
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